{"created":"2024-10-17T05:23:21.939622+00:00","id":2000937,"links":{},"metadata":{"_buckets":{"deposit":"c4f8493b-a49c-49e6-9326-2b861499f9fb"},"_deposit":{"created_by":15,"id":"2000937","owner":"15","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2000937"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:02000937","sets":["334:627:1728958044917"]},"author_link":[],"item_8_biblio_info_6":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-05-29","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本研究ではγδ型T細胞のアロ未反応性を利用したoff-the-shelf化を介して固形がんに対するCAR-T細胞療法のPOCを確立する目的で、細胞内にCD28とCD3zetaのシグナル伝達ドメインを含むCEA特異的CARならびにGITRLを同時に発現するγδ型T細胞（それぞれ、CEA.CAR-gdTとCEA.CAR-GITRL-gdT）を作成した。CEA.CAR-gdTは腫瘍縮小効果を発揮し、GITRL導入によりCEA.CAR-gdTの抗腫瘍効果はさらに増強されることを明らかにした。さらにNAC存在化にCAR-Tを調製することで、疲弊抵抗性のCAR-gdTを調製する培養法を確立した。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"Gamma/delta T cells were successfully transduced with a CAR specific to CEA with signaling domains of CD3zeta and CD28 (CEA.CAR-gd T cells). In a xenograft mouse model, CEA. CAR-gd T cells suppressed CEA+ tumor growth though a limited time window. Provision of GITR co-stimulation enhanced anti-tumor function of CEA.CAR-gd T cells, the result of which imposes additional measurers to be adopted in CAR-γδ T cells for an allogeneic adoptive immunotherapy. Therefore, CEA.CAR-gd T cells were prepared in the presence of NAC. NAC-pretreated CAR-T cells produced more IFN-g, enhanced serial killing function. These results suggest that supplementation of NAC during CAR-gd T cell preparation may be one of ways to obtain exhaustion resistant CAR-gd T cells which may have higher in vivo tumor control ability.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2020年度～2022年度科学研究費補助金(基盤研究(C))研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"20K07674","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_8_text_65":{"attribute_name":"item_8_text_65","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"加藤, 琢磨","creatorNameLang":"ja"},{"creatorName":"Kato, Takuma","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"王, 立楠","creatorNameLang":"ja"},{"creatorName":"Wang, Linan","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-10-17"}],"filename":"2024RP0028.pdf","filesize":[{"value":"112 KB"}],"format":"application/pdf","url":{"label":"2024RP0028.pdf","url":"https://mie-u.repo.nii.ac.jp/record/2000937/files/2024RP0028.pdf"},"version_id":"833b8d14-95a3-41ab-8fdc-0c64f9478203"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"キメラ抗原受容体","subitem_subject_scheme":"Other"},{"subitem_subject":"γδＴ細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"off-the-shelf","subitem_subject_scheme":"Other"},{"subitem_subject":"アロ反応性","subitem_subject_scheme":"Other"},{"subitem_subject":"移植片対宿主反応","subitem_subject_scheme":"Other"},{"subitem_subject":"抗酸化因子","subitem_subject_scheme":"Other"},{"subitem_subject":"疲弊抵抗性","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"免疫抑制回避機構を有するoff-the-shelf型CAR導入γδＴ細胞","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"免疫抑制回避機構を有するoff-the-shelf型CAR導入γδＴ細胞","subitem_title_language":"ja"},{"subitem_title":"Off-the-shelf immunotherapy with CAR-gamma delta T cells","subitem_title_language":"en"}]},"item_type_id":"8","owner":"15","path":["1728958044917"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-10-17"},"publish_date":"2024-10-17","publish_status":"0","recid":"2000937","relation_version_is_last":true,"title":["免疫抑制回避機構を有するoff-the-shelf型CAR導入γδＴ細胞"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2024-10-17T05:36:16.214687+00:00"}