{"created":"2024-10-18T04:32:44.880051+00:00","id":2000949,"links":{},"metadata":{"_buckets":{"deposit":"017b0ca9-ca00-4898-89a3-d759be06c780"},"_deposit":{"created_by":15,"id":"2000949","owner":"15","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2000949"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:02000949","sets":["334:627:1728958044917"]},"author_link":[],"item_8_biblio_info_6":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-05-29","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"CALI法とは、光照射依存的に活性酸素を放出する光増感物質を用いた分子機能不活化法である。CALI法は、in vivoでも高い特異性を有する分子操作技術であるが、分子ごとにCALIが可能な抗体をスクリーニングする必要があり、その開発には経験上約1年を要する。よって様々な神経伝達物質受容体に対するCALI法を次々に開発することは、現在のところ極めて困難である。本研究ではこの解決を目指し、我々が開発したCALI効率を迅速に最適化する新手法（ハイスループットCALI法）を様々な分子に適用したところ、主要な膜タンパク質に対するCALI法をハイスループットに開発できることを実証した。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"The CALI method is a molecular function inactivation method using photosensitizers that release reactive oxygen species in a light-irradiation-dependent manner. It is currently extremely difficult to develop CALI methods for various neurotransmitter receptors one after another. In this study, we applied our new method for rapid optimization of CALI efficiency (high-throughput CALI method) to various molecules and successfully demonstrated the development of high-throughput CALI methods for major membrane proteins.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2021年度～2022年度科学研究費補助金(挑戦的研究(萌芽))研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"21K19311","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_8_text_65":{"attribute_name":"item_8_text_65","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"竹本, 研","creatorNameLang":"ja"},{"creatorName":"Takemoto, Kiwamu","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-10-18"}],"filename":"2024RP0040.pdf","filesize":[{"value":"78 KB"}],"format":"application/pdf","url":{"label":"2024RP0040.pdf","url":"https://mie-u.repo.nii.ac.jp/record/2000949/files/2024RP0040.pdf"},"version_id":"d243b290-2fe3-4be4-8b45-ae8c00f98867"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"光操作","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"シナプス分子の光操作に資する革新的技術プラットフォームの創生","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"シナプス分子の光操作に資する革新的技術プラットフォームの創生","subitem_title_language":"ja"},{"subitem_title":"A technical platform for optical manipulation of synaptic molecules","subitem_title_language":"en"}]},"item_type_id":"8","owner":"15","path":["1728958044917"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-10-18"},"publish_date":"2024-10-18","publish_status":"0","recid":"2000949","relation_version_is_last":true,"title":["シナプス分子の光操作に資する革新的技術プラットフォームの創生"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2024-10-18T04:41:23.990381+00:00"}