{"created":"2024-10-22T00:40:27.426113+00:00","id":2000952,"links":{},"metadata":{"_buckets":{"deposit":"fa5f7ee6-36a6-497b-8fd9-3a403d81f70e"},"_deposit":{"created_by":15,"id":"2000952","owner":"15","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2000952"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:02000952","sets":["334:627:1728958044917"]},"author_link":[],"control_number":"2000952","item_8_biblio_info_6":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-06-12","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ヒト臍帯静脈血管内皮細胞 HUVECに、IL-1β/TGF-β2を添加し、HUVECのEndMTが誘導される。HUVECEndMTedと前立腺癌上皮（LNCaP）を共培養することで、共培養したLNCaPにおいて神経内分泌化マーカーのChromogranin Aの発現上昇を認めた。共培養後のLNCaPの遺伝子網羅的解析において、Synaptophysinなどの他の神経内分泌化マーカーの上昇もみられた。ヒト神経内分泌化前立腺癌検体において、血管内皮細胞は神経内分泌化した前立腺癌細胞と近接していることがわかった。EndMTを誘導したHUVECは、LNCaPの神経内分泌化を促進する可能性が示された。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"Endothelial-mesenchymal transition (EndMT) has been implicated in the malignant transformation of various tumors. Endothelial and tumor cells with neuroendocrine differentiation (NED) were found to be in close proximity in human prostate cancer (PC). This study investigates whether the EndMT of endothelial cells in the vicinity of cancer cells contributes to the NED of PC. We induced EndMT in HUVEC by IL-1b and TGF-b2 and co-cultured them with LNCaP. Then we evaluated the expression of neuroendocrine markers and cell proliferation of LNCaP. We comprehensively analyzed the gene expression of EndMTed HUVEC to identify factors inducing NED of LNCaP.When co-cultured with EndMTed HUVEC, LNCaP exhibited increased expression of neuroendocrine markers and cell proliferation. Several genes with significantly upregulated expression in EndMTed HUVEC were indicated as involved in the NED.　Thus, EndMT of endothelial cells in the prostate microenvironment may play a role in the NED of PC.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2019年度～2022年度科学研究費補助金(若手研究)研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"19K18556","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_8_text_65":{"attribute_name":"item_8_text_65","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"加藤, 学","creatorNameLang":"ja"},{"creatorName":"Kato, Manabu","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-10-22"}],"filename":"2024RP0043.pdf","filesize":[{"value":"82 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"2024RP0043.pdf","url":"https://mie-u.repo.nii.ac.jp/record/2000952/files/2024RP0043.pdf"},"version_id":"73686606-f99f-44a3-9d4e-87b1222d2710"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"前立腺癌","subitem_subject_scheme":"Other"},{"subitem_subject":"血管内皮細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"血管内皮間葉移行","subitem_subject_scheme":"Other"},{"subitem_subject":"神経内分泌分化","subitem_subject_scheme":"Other"},{"subitem_subject":"ホルモン療法","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"前立腺癌の去勢抵抗性獲得におけるCD105が関連する内皮間葉分化転換の役割","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"前立腺癌の去勢抵抗性獲得におけるCD105が関連する内皮間葉分化転換の役割","subitem_title_language":"ja"},{"subitem_title":"Endothelial-mesenchymal transition promotes the neuroendocrine differentiation of prostate cancer","subitem_title_language":"en"}]},"item_type_id":"8","owner":"15","path":["1728958044917"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-10-22"},"publish_date":"2024-10-22","publish_status":"0","recid":"2000952","relation_version_is_last":true,"title":["前立腺癌の去勢抵抗性獲得におけるCD105が関連する内皮間葉分化転換の役割"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2024-10-22T01:07:34.017541+00:00"}