{"created":"2024-10-22T02:30:24.868168+00:00","id":2000957,"links":{},"metadata":{"_buckets":{"deposit":"37369fae-7da4-46bb-a76b-90fa6a611ac5"},"_deposit":{"created_by":15,"id":"2000957","owner":"15","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2000957"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:02000957","sets":["334:627:1728958044917"]},"author_link":[],"control_number":"2000957","item_8_biblio_info_6":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-06-08","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"象牙芽細胞特異的遺伝子Dspp及びエナメル芽細胞特異的遺伝子Amelxの発現をGFP及びtdTomatoで検出できるDspp-GFPマウス及びAmelx-tdTomatoマウス、二つの遺伝子改変マウスよりDspp-GFP； Amelx-tdTomatoマウスを作成し、象牙芽細胞及びエナメル芽細胞を別蛍光を指標に組織学的及びフローサイトメトリーにて同定・単離した。これら二重遺伝子組み換えマウスから胚性幹細胞株を樹立し、別蛍光を指標に試験管内で象牙芽細胞とエナメル芽細胞の誘導を試みている。これらのマウスから培養前は陰性で培養後に蛍光陽性になる象牙芽細胞或いはエナメル芽細胞の前駆細胞を同定している。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"We established Dspp-GFP; Amelx-tdTomato double transgenic mice, that enable us to detect odontoblasts and ameloblasts as GFP-expressing cells or tdTomato-expressing cells fluorescently. Using these mice, GFP+ odontoblasts and tdtomato+ ameloblasts were identified and isolated by immunohistochemistry and flow cytometry. Furthermore, we have established embryonic stem cell lines from Dspp-GFP; Amelx-tdTomato double transgenic mice and attempted to induce GFP+ odontoblasts and tdTomato+ ameloblasts in vitro using different fluorescence markers.\nIn addition, from these mice, we have identified progenitor cells of odontoblasts or ameloblasts that are negative before culture but become positive for fluorescence after culture by using tooth organ culture.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2019年度～2022年度科学研究費補助金(基盤研究(C))研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"19K10041","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_8_text_65":{"attribute_name":"item_8_text_65","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山崎, 英俊","creatorNameLang":"ja"},{"creatorName":"Yamazaki, Hidetoshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"礒野, 加奈","creatorNameLang":"ja"},{"creatorName":"Isono, Kana","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-10-22"}],"filename":"2024RP0048.pdf","filesize":[{"value":"786 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"2024RP0048.pdf","url":"https://mie-u.repo.nii.ac.jp/record/2000957/files/2024RP0048.pdf"},"version_id":"b72df560-1451-41f5-9005-58d85c9f38cd"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"エナメル芽細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"象牙芽細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"Amelogenin-X","subitem_subject_scheme":"Other"},{"subitem_subject":"Dspp","subitem_subject_scheme":"Other"},{"subitem_subject":"前駆細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"tdTomato","subitem_subject_scheme":"Other"},{"subitem_subject":"GFP","subitem_subject_scheme":"Other"},{"subitem_subject":"胚性幹細胞","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"象牙芽細胞とエナメル芽細胞を蛍光標識できるマウスの歯の前駆細胞を用いた歯の形成","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"象牙芽細胞とエナメル芽細胞を蛍光標識できるマウスの歯の前駆細胞を用いた歯の形成","subitem_title_language":"ja"},{"subitem_title":"Tooth formation using mouse tooth progenitor cells that can fluorescently label odontoblasts and ameloblasts.","subitem_title_language":"en"}]},"item_type_id":"8","owner":"15","path":["1728958044917"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-10-22"},"publish_date":"2024-10-22","publish_status":"0","recid":"2000957","relation_version_is_last":true,"title":["象牙芽細胞とエナメル芽細胞を蛍光標識できるマウスの歯の前駆細胞を用いた歯の形成"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2024-10-22T02:42:59.880387+00:00"}