{"created":"2024-10-23T02:06:38.184788+00:00","id":2000970,"links":{},"metadata":{"_buckets":{"deposit":"ab9637bc-2263-4a15-84dd-9e37a95369ba"},"_deposit":{"created_by":15,"id":"2000970","owner":"15","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2000970"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:02000970","sets":["631:636:1728958087061"]},"author_link":[],"item_8_biblio_info_6":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-06-05","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"αβ-TCRとCD8を発現する遺伝子改変γδT細胞（GMC）は、抗原特異的細胞傷害性と優れた持続性を示した。GMCのサイトカイン分泌は非遺伝子改変γδ-T細胞（NGMC）よりもATP合成酵素阻害剤により強く阻害された。メタボロームおよびトランスクリプトーム解析により、GMCはTCAサイクルと酸化的リン酸化をより利用していることが明らかになった。細胞外フラックスによるOCR/ECAR比は、GMCのミトコンドリア機能の利用が高いことを支持していた。\n以上より、αβ-TCRを遺伝子導入したγδ-T細胞は、ミトコンドリアのエネルギー代謝を優先的に利用し、優れた持続性をもたらすと結論付けた。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"Genetically modified Vγ9+Vδ2+ T cells expressing a tumor antigen-specific αβ-TCR and CD8 coreceptor (GMC) showed target-specific killing and excellent persistence. To clarify the mechanisms, we investigated the metabolic characteristics of GMC. Cytokine secretion of αβ-TCR-stimulated GMCs was more strongly inhibited by ATP synthase inhibitors than that of γδ -TCR-stimulated nongene-modified γδ-T cells (NGMCs). Metabolomic and transcriptomic analyses revealed that GMCs utilize the TCA cycle and oxidative phosphorylation more than NGMCs. The higher oxygen consumption rate-to-extracellular acidification rate ratio by extracellular flux analysis supports higher utilization of mitochondrial oxidative metabolism in αβ-TCR-stimulated GMCs. In conclusion, γδ-T cells transduced with αβ-TCR use mitochondrial energy metabolism preferentially, leading to excellent persistence.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2020年度～2022年度科学研究費補助金(基盤研究(C))研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"20K07656","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_8_text_65":{"attribute_name":"item_8_text_65","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"石原, 幹也","creatorNameLang":"ja"},{"creatorName":"Ishihara, Mikiya","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"三輪, 啓志","creatorNameLang":"ja"},{"creatorName":"Miwa, Hiroshi","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-10-23"}],"filename":"2024RP0060.pdf","filesize":[{"value":"80 KB"}],"format":"application/pdf","url":{"label":"2024RP0060.pdf","url":"https://mie-u.repo.nii.ac.jp/record/2000970/files/2024RP0060.pdf"},"version_id":"08dfbde0-bc6b-4d24-b275-5ea0a1e940c0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"γδT細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"αβ-TCR","subitem_subject_scheme":"Other"},{"subitem_subject":"ミトコンドリア機能","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"エネルギー代謝に注目した多機能遺伝子改変γδT細胞の追求","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"エネルギー代謝に注目した多機能遺伝子改変γδT細胞の追求","subitem_title_language":"ja"},{"subitem_title":"Pursuit of multifunctional gene-modified gamma-delta T cells focusing on energy metabolism","subitem_title_language":"en"}]},"item_type_id":"8","owner":"15","path":["1728958087061"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-10-23"},"publish_date":"2024-10-23","publish_status":"0","recid":"2000970","relation_version_is_last":true,"title":["エネルギー代謝に注目した多機能遺伝子改変γδT細胞の追求"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2024-10-23T02:22:20.962367+00:00"}