{"created":"2024-10-25T04:02:46.549346+00:00","id":2001002,"links":{},"metadata":{"_buckets":{"deposit":"b05b2334-2499-4bfc-9278-248607450421"},"_deposit":{"created_by":15,"id":"2001002","owner":"15","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2001002"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:02001002","sets":["366:680:1728958155548"]},"author_link":[],"item_8_biblio_info_6":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-06-05","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"本研究の遂行のため基本技術の改良として１）逆相遠心法で大量調製したGUVへのバキュロウイルス出芽粒子（BV）の膜融合をレーザー共焦点顕微鏡で観察し融合効率が向上する溶媒選択、脂質組成を検討し、特にPEが補助因子として働くことを見出し、球状固体粒子（シリカビーズ）膜担持形成で得た球状支持調製への組換えタンパク質導入を解析した。２）細胞接着関連タンパク質を膜へ導入に資するため、BV保存法の解析、組換えFERMタンパク質(RDX)のBVとの挙動を解析した。３）細胞接着タンパク質によるGUV集積機能の発現を確認した。最後に４）水性二相系微小液滴に細胞骨格（アクチン）等の取込（分配）挙動を検討した。","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"To achieve the constitution of artificial cell assemblies, we’ve basically investigated the following experimental concerns. 1) Membrane fusion between baculovirus budded virions (BVs) and GUVs prepared using the reverse-phase/centrifugation method were analyzed and phosphatidylethanolamine was found to serve as cofactor promoting the fusion. We developed lipid-membrane coated silica microbeads containing recombinant proteins introduced by BVs. 2) Expression of cell adhesion proteins and FERM proteins on BVs were investigated. 3) GUV assembly were able to be induced by the aforesaid adhesive proteins. 4) Aqueous/aqueous microdroplets in an aqueous two-phase system (ATPS) could be used for an efficient vessel concentrating cytoskeletal proteins.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2019年度～2022年度科学研究費補助金(基盤研究(C))研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"19K06540","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_8_text_65":{"attribute_name":"item_8_text_65","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"湊元, 幹太","creatorNameLang":"ja"},{"creatorName":"Tsumoto, Kanta","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"瀧口, 金吾","creatorNameLang":"ja"},{"creatorName":"Takiguchi, Kingo","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-10-25"}],"filename":"2024RP0092.pdf","filesize":[{"value":"295 KB"}],"format":"application/pdf","url":{"label":"2024RP0092.pdf","url":"https://mie-u.repo.nii.ac.jp/record/2001002/files/2024RP0092.pdf"},"version_id":"7ef51aa9-ffea-4688-82b7-87c6f8f030e5"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"人工細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"リポソーム","subitem_subject_scheme":"Other"},{"subitem_subject":"GUV","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞骨格","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞接着","subitem_subject_scheme":"Other"},{"subitem_subject":"バキュロウイルス","subitem_subject_scheme":"Other"},{"subitem_subject":"脂質二分子膜","subitem_subject_scheme":"Other"},{"subitem_subject":"マイクロコンパ ートメント","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"細胞結合ネットワークの構築による人工細胞モデルの組織化と集団動態発現","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"細胞結合ネットワークの構築による人工細胞モデルの組織化と集団動態発現","subitem_title_language":"ja"},{"subitem_title":"Organization of artificial cells into an assembled model that can express orchestrated dynamic behavior through cell junction networks","subitem_title_language":"en"}]},"item_type_id":"8","owner":"15","path":["1728958155548"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-10-25"},"publish_date":"2024-10-25","publish_status":"0","recid":"2001002","relation_version_is_last":true,"title":["細胞結合ネットワークの構築による人工細胞モデルの組織化と集団動態発現"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2024-10-25T04:10:25.605338+00:00"}