{"created":"2024-10-28T00:48:03.150568+00:00","id":2001010,"links":{},"metadata":{"_buckets":{"deposit":"c3185dad-949f-49d4-ba86-f178529b50f4"},"_deposit":{"created_by":15,"id":"2001010","owner":"15","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2001010"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:02001010","sets":["420:724:1728958246114"]},"author_link":[],"item_8_biblio_info_6":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2023-06-12","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"4-deoxy-L-erythro-5-hexoseulose uronic acid（DEH）は，アルギン酸からエキソ型アルギン酸リアーゼの作用によって生じる唯一の単糖（アルギン酸デオキシ糖）である．この研究では，アルギン酸を含む食品の分析に適用出来るDEHをスタンダードとした新しいアルギン酸の定量分析法を開発することを目的とした．次の二つの研究成果を得た．①アルギン酸分解・資化性細菌ライブラリーからアルギン酸リアーゼを選抜し，アルギン酸をDEHへと完全分解出来るベストミックス酵素を開発した，②LC/MS法によるDEHの検出と分析精度の評価に関する試験し，アルギン酸の定量分析法を決定した．","subitem_description_language":"ja","subitem_description_type":"Abstract"},{"subitem_description":"4-Deoxy-L-erythro-5-hexoseulose uronic acid (DEH) is the only monosaccharide (alginate deoxysugar) produced from alginic acid by the action of exo-alginate lyases. The purpose of this study was to develop a new quantitative analysis method for alginic acid using DEH as a standard, which can be applied to the analysis of foods containing alginic acid. The following two research results were obtained:(1) alginate lyase was selected from the alginate-degrading and assimilating bacterial library, and the best-mixed enzyme capable of completely degrading alginate to DEH was developed, and (2) a quantitative analysis method for alginic acid was completed by testing the detection of DEH by the LC/MS method and the evaluation of analytical accuracy.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2020年度～2022年度科学研究費補助金(基盤研究(C))研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"20K02344","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_8_text_65":{"attribute_name":"item_8_text_65","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"柴田, 敏行","creatorNameLang":"ja"},{"creatorName":"Shibata, Toshiyuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"田中, 礼士","creatorNameLang":"ja"},{"creatorName":"Tanaka, Reiji","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-10-28"}],"filename":"2024RP0100.pdf","filesize":[{"value":"116 KB"}],"format":"application/pdf","url":{"label":"2024RP0100.pdf","url":"https://mie-u.repo.nii.ac.jp/record/2001010/files/2024RP0100.pdf"},"version_id":"b6a44e7c-7513-4125-bfb3-2ad243e4c14b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"アルギン酸","subitem_subject_scheme":"Other"},{"subitem_subject":"アルギン酸リアーゼ","subitem_subject_scheme":"Other"},{"subitem_subject":"アルギン酸デオキシ糖","subitem_subject_scheme":"Other"},{"subitem_subject":"DEH","subitem_subject_scheme":"Other"},{"subitem_subject":"定量分析","subitem_subject_scheme":"Other"},{"subitem_subject":"LC/MS","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"アルギン酸完全分解酵素を用いた新しいアルギン酸定量分析法の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"アルギン酸完全分解酵素を用いた新しいアルギン酸定量分析法の開発","subitem_title_language":"ja"},{"subitem_title":"Development of a new method for quantitative analysis of alginate using complete alginate degrading enzyme","subitem_title_language":"en"}]},"item_type_id":"8","owner":"15","path":["1728958246114"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-10-28"},"publish_date":"2024-10-28","publish_status":"0","recid":"2001010","relation_version_is_last":true,"title":["アルギン酸完全分解酵素を用いた新しいアルギン酸定量分析法の開発"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2024-10-28T00:57:35.099576+00:00"}