{"created":"2025-05-19T05:52:56.982598+00:00","id":2001468,"links":{},"metadata":{"_buckets":{"deposit":"ffe22e03-4e87-4a99-b543-7753a6d39904"},"_deposit":{"created_by":15,"id":"2001468","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"2001468"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:02001468","sets":["334:608:618:1721969789237"]},"author_link":[],"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2025-03-25","bibliographicIssueDateType":"Issued"}}]},"item_7_date_granted_59":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2025-03-25"}]},"item_7_degree_grantor_57":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"三重大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"14101","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_7_degree_name_56":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)"}]},"item_7_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"(1) Background: Exosomal PD-L1 has garnered attention owing to its role in instigating systemic immune suppression. The objective of this study is to elucidate whether bone and soft tissue sarcoma cells possess the capacity to secrete functionally active exosomal PD-L1 and whether radiotherapy (RT) induces the exosomal PD-L1 release. (2) Methods: Human osteosarcoma cell line 143B and human fibrosarcoma cell line HT1080 were utilized. Exosomes were isolated from the culture medium and blood via ultracentrifugation. The expression of PD-L1 on both tumor cells and exosomes was evaluated. The inhibitory effect on PBMC was employed to assess the activity of exosomal PD-L1. Post radiotherapy, changes in PD-L1 expression were compared. (3) Results: Exosomal PD-L1 was detected in the culture medium of tumor cells but was absent in the culture medium of PD-L1 knockout cells. Exosomal PD-L1 exhibited an inhibitory effect on PBMC activation. In tumor-bearing mice, human-derived exosomal PD-L1 was detected in the bloodstream. Following radiotherapy, tumor cells upregulated PD-L1, and human-derived exosomal PD-L1 were detected in the bloodstream. (4) Conclusions: Exosomal PD-L1 emanates from bone and soft tissue sarcoma cells and is disseminated into the circulatory system. The levels of PD-L1 in tumor cells and the release of exosomal PD-L1 were augmented after irradiation with RT.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_7_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"本文 / Department of Orthopaedic Surgery, Mie University Graduate School of Medicine, Tsu 514-8507, Mie, Japan","subitem_description_type":"Other"},{"subitem_description":"14p","subitem_description_type":"Other"}]},"item_7_dissertation_number_60":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"甲医学第2314号"}]},"item_7_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_7_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.3390/cancers16132489","subitem_relation_type_select":"DOI"}}]},"item_7_text_31":{"attribute_name":"出版者（ヨミ）","attribute_value_mlt":[{"subitem_text_value":"ミエダイガク"}]},"item_7_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Doctoral Dissertation / 博士論文"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"吉田, 圭佑","creatorNameLang":"ja"},{"creatorName":"ヨシダ, ケイスケ","creatorNameLang":"ja-Kana"},{"creatorName":"Yoshida, Keisuke","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"2024DM0338.pdf","filesize":[{"value":"8.25 MB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"label":"2024DM0338.pdf","objectType":"fulltext","url":"https://mie-u.repo.nii.ac.jp/record/2001468/files/2024DM0338.pdf"},"version_id":"b8d6ae36-91a5-4d80-9b36-82aa95be9991"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"programmed death-ligand 1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"soft tissue","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"bone","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"sarcoma","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"exosome","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Release of Exosomal PD-Ll in Bone and Soft Tissue Sarcomas and Its Relationship to Radiotherapy","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Release of Exosomal PD-Ll in Bone and Soft Tissue Sarcomas and Its Relationship to Radiotherapy","subitem_title_language":"en"}]},"item_type_id":"7","owner":"15","path":["1721969789237"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-05-19"},"publish_date":"2025-05-19","publish_status":"0","recid":"2001468","relation_version_is_last":true,"title":["Release of Exosomal PD-Ll in Bone and Soft Tissue Sarcomas and Its Relationship to Radiotherapy"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2025-05-19T06:49:42.590678+00:00"}