{"created":"2023-06-19T11:36:05.507458+00:00","id":2931,"links":{},"metadata":{"_buckets":{"deposit":"bae12b9c-6ff1-4fe0-9480-275823879125"},"_deposit":{"created_by":13,"id":"2931","owners":[13],"pid":{"revision_id":0,"type":"depid","value":"2931"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00002931","sets":["143:144:234:242"]},"author_link":["37443","37444"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1985-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"102","bibliographicPageStart":"91","bibliographicVolumeNumber":"36","bibliographic_titles":[{"bibliographic_title":"三重大学教育学部研究紀要. 自然科学"}]}]},"item_4_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"DNA合成阻害剤（5-aminouracil）を用いて、ソラマメ（Vicia faba）根端細胞の高い同調分裂を誘導し、これから酵素的にプロトプラストを遊離した。　(1)根端細胞での同調分裂の誘導は下記の処理条件で高い分裂指数（44％）が得られた。　阻害剤濃度500ppm、19℃、11時間　阻害剤除去後　25℃、15時間目　(2)同調分裂させた根端細胞から下記の酵素処理条件下で核分裂中のプロトプラストがいくらか（分裂指数17％）得られた。　酵素組成　0.10％　Pectolyase Y-23　1.00％　Driselase　2.00％　Cellulase onozuka RS　0.25％　mannitol　（pH5.50）　処理条件　ソラマメの根端をカミソリで薄く切る。　3.0ml/50ml三角フラスコ　25℃往復振盪120（rpm）30分間処理後酵素液を替えてさらに30分間処理する。","subitem_description_type":"Abstract"}]},"item_4_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学教育学部"}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0389-9225","subitem_source_identifier_type":"PISSN"}]},"item_4_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00234199","subitem_source_identifier_type":"NCID"}]},"item_4_text_18":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Isolation of Protoplasts from Vicia faba Root Meristematic Tissues Treated with 5-Aminouracil"}]},"item_4_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper / 紀要論文"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"臼井, 英夫","creatorNameLang":"ja"},{"creatorName":"Usui, Hideo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"37443","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"後藤, 安代","creatorNameLang":"ja"},{"creatorName":"Goto, Yasuyo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"37444","nameIdentifierScheme":"WEKO"}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"5-アミノウラシル処理をしたソラマメの根の分裂組織からのプロトプラストの遊離","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"5-アミノウラシル処理をしたソラマメの根の分裂組織からのプロトプラストの遊離","subitem_title_language":"ja"}]},"item_type_id":"4","owner":"13","path":["242"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-03-16"},"publish_date":"2018-03-16","publish_status":"0","recid":"2931","relation_version_is_last":true,"title":["5-アミノウラシル処理をしたソラマメの根の分裂組織からのプロトプラストの遊離"],"weko_creator_id":"13","weko_shared_id":-1},"updated":"2023-11-07T02:02:14.387339+00:00"}