{"created":"2023-06-19T11:36:12.357277+00:00","id":3076,"links":{},"metadata":{"_buckets":{"deposit":"47562b1f-7f64-4aa6-a281-b6bd828e8b82"},"_deposit":{"created_by":13,"id":"3076","owners":[13],"pid":{"revision_id":0,"type":"depid","value":"3076"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00003076","sets":["143:144:234:252"]},"author_link":["37771","37772"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995-03-15","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"68","bibliographicPageStart":"57","bibliographicVolumeNumber":"46","bibliographic_titles":[{"bibliographic_title":"三重大学教育学部研究紀要. 自然科学"}]}]},"item_4_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"An investigation was made on the adaptation of Lowry's method, Somogyi-Neison's method and Bragdon's method to the micro-determination of protein, sugar and lipid concentrations in human milk. The result was: Lowry's method enabled the micro-determination of protain concentrations by 50 μl of human milk; Somogyi-Nelson's method that of sugar concentrations by 25 μl; Bragdon's method thet of lipid concentrations by 100 μl. Calibration curves,recovery rates and coefficients of variation confirmed that these methods were reproductive. Saving reagents and simplifying procedures, each method enabled the simultaneous measurement of samples.@@@母乳中たんぱく質・糖質・脂質の微量定量法のために、Lowry法・Somogyi-Nelson法・Bragdonの酸化比色法について、検討を行った。たんぱく質はLowry法によって、母乳50μlで定量できることが明らかになった。また、本法でのたんぱく質定量の上限は100μlであった。糖質はNelson法によって、母乳25μlで定量できることが明らかになった。また、本法でのラクトース定量の上限は100μlであった。脂質はBragdon法によって、母乳100μlで定量できることが明らかになった。また、本法での脂質定量の上限は1.1mg以下と推測された。これらの三法とも、検量線、定量値のCV値、添加回収実験によって再現性のある定量法であることが確かめられた。また、各法ともに試薬の節約や操作の簡便化をはかり、同時に多数の試料を定量可能にした。","subitem_description_type":"Abstract"}]},"item_4_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学教育学部"}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0389-9225","subitem_source_identifier_type":"PISSN"}]},"item_4_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00234199","subitem_source_identifier_type":"NCID"}]},"item_4_text_18":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Micro-determination of Protein, Sugar and Lipid Concentrations in Human Milk"}]},"item_4_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper / 紀要論文"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"成田, 美代","creatorNameLang":"ja"},{"creatorName":"Narita, Miyo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"大嶽, 紀子","creatorNameLang":"ja"},{"creatorName":"Ohtake, Noriko","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"母乳中たんぱく質・糖質・脂質の微量定量法について","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"母乳中たんぱく質・糖質・脂質の微量定量法について","subitem_title_language":"ja"}]},"item_type_id":"4","owner":"13","path":["252"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-03-16"},"publish_date":"2018-03-16","publish_status":"0","recid":"3076","relation_version_is_last":true,"title":["母乳中たんぱく質・糖質・脂質の微量定量法について"],"weko_creator_id":"13","weko_shared_id":-1},"updated":"2023-11-07T05:36:26.785388+00:00"}