{"created":"2023-06-19T11:37:38.238934+00:00","id":5021,"links":{},"metadata":{"_buckets":{"deposit":"fc463f8a-adb7-4aca-a249-04d789c44b86"},"_deposit":{"created_by":13,"id":"5021","owners":[13],"pid":{"revision_id":0,"type":"depid","value":"5021"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00005021","sets":["366:367:368:390"]},"author_link":["10777","10778","10779","10780"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1997-12-24","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"77","bibliographicPageStart":"69","bibliographicVolumeNumber":"22","bibliographic_titles":[{"bibliographic_title":"Research reports of the Faculty of Engineering, Mie University"}]}]},"item_4_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"All creatures, including procaryotes, harbor genetic information in deoxyribonucleic acid (DNA). Some viruses, however, store their genes in the form of ribonucleic acid (RNA) instead of DNA. To elucidate the functions of RNA viruses, determination of the nucleotide sequences making up their genetic material is necessary. However, direct sequencing of RNA is at present impossible. To address this problem, synthesis of complementary DNA (cDNA) using reverse transcriptase is generally applied. The problem is that the efficiency of this reverse reaction is extremely low. We have therefore employed the polymerase chain reaction to amplify cDNA after reverse transcription, and could thereby successfully determine the complete nucleotide sequence of a polyhedrin gene of Bombyx mori cytoplasmic polyhedrosis virus, which harbors its genome as double-stranded RNA.","subitem_description_type":"Abstract"}]},"item_4_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Faculty of Engineering, Mie University"}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-6208","subitem_source_identifier_type":"PISSN"}]},"item_4_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA00816341","subitem_source_identifier_type":"NCID"}]},"item_4_text_18":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"2本鎖RNA遺伝子のクローニングと塩基配列決定"}]},"item_4_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper / 紀要論文"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tomita, Masahiro","creatorNameLang":"en"},{"creatorName":"冨田, 昌弘","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"10777","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Miyajima, Shigetoshi","creatorNameLang":"en"},{"creatorName":"宮嶌, 成壽","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"10778","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshimura, Tetsuro","creatorNameLang":"en"},{"creatorName":"吉村, 哲郎","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"10779","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suzuki, Yoshiaki","creatorNameLang":"en"},{"creatorName":"鈴木, 義昭","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"10780","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-18"}],"displaytype":"detail","filename":"AA008163410220008.PDF","filesize":[{"value":"458.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AA008163410220008.PDF","url":"https://mie-u.repo.nii.ac.jp/record/5021/files/AA008163410220008.PDF"},"version_id":"bcae7d3b-5e17-4062-af32-8afb437a3692"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"reverse transcription","subitem_subject_scheme":"Other"},{"subitem_subject":"polymerase chain reaction","subitem_subject_scheme":"Other"},{"subitem_subject":"double-stranded RNA","subitem_subject_scheme":"Other"},{"subitem_subject":"polyhedrin gene","subitem_subject_scheme":"Other"},{"subitem_subject":"point-mutation","subitem_subject_scheme":"Other"},{"subitem_subject":"Bombyx mori cytoplasmic polyhedrosis virus","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Cloning and Sequencing of Double-Stranded RNA","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cloning and Sequencing of Double-Stranded RNA","subitem_title_language":"en"}]},"item_type_id":"4","owner":"13","path":["390"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-07-02"},"publish_date":"2007-07-02","publish_status":"0","recid":"5021","relation_version_is_last":true,"title":["Cloning and Sequencing of Double-Stranded RNA"],"weko_creator_id":"13","weko_shared_id":-1},"updated":"2023-10-05T06:57:09.443020+00:00"}