@article{oai:mie-u.repo.nii.ac.jp:00005637,
 author = {竹内, 俊博 and Takeuchi, Toshihiro and 宮崎, 照雄 and Miyazaki, Teruo},
 journal = {三重大学水産学部研究報告 = Bulletin of the Faculty of Fisheries, Mie University},
 month = {Nov},
 note = {application/pdf, Both Aeromonas hydrophila and Aeromonas salmonicida were injected into the skin of carps(23g in mean body weight) with 0.1ml of PBS containing A. hydrophila 10⁶ CFU＋ A. salmonicida 10⁶ CFU /ml, A. hydrophila 10⁷ CFU＋ A. salmonicida 10⁷ CFU/ml, A. hydrophila 10⁶ CFU＋ A. salmonicida 10⁸ CFU/ml and A.hydrophila 10⁵ CFU＋A. salmonicida 10⁹ CFU/ml. The injected lesions were studied with histopathological and indirect fluorescent antibody techniques (IFAT). All injected lesions showed necrosis,hemorrhage and bacterial multiplication in the skin and lateral musculature. IFAT used the absorbed anti-A.hydrophila-serum markedly revealed fluorescent antibody response in all injected lesions, indicating the bacterial multiplication in tissues. On the other hand,IFAT used the absorbed anti-A.salmonicida-serum narrowly revealed fluorescent antibody response in the lesions in which A.hydrophila 10⁵ CFU＋A.salmonicida 10⁷ CFU and  A.hydrophila 10⁴ CFU＋A. salmonicida 10⁸ CFU were injected. These results indicated that A.hydrophila more dominantly multiplied in the carp tissues than A.salmonicida when they were mixed. The indirect fluorescent antibody technique used absorbed antisera proved to be an effective and useful method for bacterial distinction in lesions where bacteria belonging to the same genus were mixed.},
 pages = {145--150},
 title = {蛍光抗体法によるコイ組織内のエロモナス菌の検出},
 volume = {13},
 year = {1986}
}