@article{oai:mie-u.repo.nii.ac.jp:00005643,
 author = {Miyazaki, Teruo and 宮崎, 照雄 and Kurata, Keikichi and 倉田, 恵吉},
 journal = {三重大学水産学部研究報告 = Bulletin of the Faculty of Fisheries, Mie University},
 month = {Sep},
 note = {application/pdf, We studied histopathological features of the Japanese eel Anguilla japonica that were experimentally infected by the intraperitoneal injection with attenuated and virulent Vibrio unlnificus (3.2×10⁷ CFU/100g body weight), and also studied the vitro phagocytic activity of peritoneal exudate cells (PEC) of eels, unvaccinated and vaccinated with the sonicated bacterin, against the viable bacteria. Eels that were infected with virulent bacteria were moribund within 24 hrs, and showed the systemic infection followed by necrosis and hemorrhage in visceral organs. Eels that were infected with attenuated bacteria survived for 7 days and showed bacterial phagocytosis of the peritoneal exudate cells, and slight necrosis and hemorrhage with bacterial invasions in the intestine on the third day. 
PEC that were induced by intraperitoneal injection with protease peptone solution, contained macrophages and neutrophils. PEC of unvaccinated fish showed slight phagocytosis against attenuated bacteria but heavy phagocytosis and intracytoplasmic multiplication of virulent bacteria. PEC of vaccinated fish displayed significantly increased phagocytic activity against attenuated bacteria on the 7th and 14th days. They showed significantly increased phagocytic activity against virulent bacteria on the 14th day, and the preventive activity of bacterial intracytoplasmic multiplication on 7th day and it was significantly increased on 14th day.},
 pages = {33--40},
 title = {Phagocytic Response of Peritoneal Exudate Cells of the Japanese Eel against Vibrio vulnificus},
 volume = {14},
 year = {1987}
}