@article{oai:mie-u.repo.nii.ac.jp:00005678,
 author = {Kashiwagi, Masaaki and 柏木, 正章 and Iwai, Toshio and 岩井, 寿夫 and Lopes, Alexandre N.G.},
 journal = {三重大学生物資源学部紀要 = The bulletin of the Faculty of Bioresources, Mie University},
 month = {Dec},
 note = {application/pdf, Responses of eggs of the pond smelt Hypomesus olidus to a series of temperature and chlorinity combinations were measured for percentages of total-and viable-hatch and time to 50% hatch.
 Hatch occurred at the combinations of temperature range of 10 to 22.5℃ and chlorinity range of 0 to 10‰. The relationships between hatching rate and two variables were expressed by the second order equations:
 TH=37.9974＋6.2171T-<special>0.3220T2</special>-3.1775C-<special>0.6297C2</special>＋0.3355TC
 VH=55.4947＋3.1276T-<special>0.2344T2</special>-5.3836C-<special>0.5386C2</special>＋0.4340TC
where TH=total-hatch (%),VH=viable-hatch(%),T=temperature(℃),C=chlorinity(‰). Response surface analysis suggests that the viable-hatch occurred safely at temperature lower than 19.0℃ and at chlorinity lower than 6.7‰.
 Time required to 50% hatch varied between 7.3 and 23.3 days after fertilization,and was inversely and exponentially related to the temperature. The effect of chlorinity on the time was statistically insignificant within the hachable range.The relationship equation was third order:
 D=131.3675-19.5853T ＋<special>1.0729T2</special>-<special>0.0202T3</special>
where D= days to 50% hatch., ワカサギの受精卵を水温７段階（範囲10～25℃, 間隔2.5℃）と塩素量６段階（範囲0～10‰, 間隔2‰)からなる42組の試水中で孵化させた。
その結果, 孵化がみられた水温と塩素量はそれぞれ22.5℃以下および10‰以下であった。総孵化率TH（%）, 正常孵化率VH(%), 水温T(℃), 塩素量C(‰)の関係は次の2次式で表された。
TH=37.9974＋6.2171T-<special>0.3220T2</special>-3.1775C-<special>0.6297C2</special>＋0.3355TC
VH=55.4947＋3.1276T-<special>0.2344T2</special>-5.3836C-<special>0.5386C2</special>＋0.4340TC
これらの式から作られた応答曲面によれば, 供試卵の半数が正常に孵化する水温と塩素量の上限はそれぞれ19℃および6.7‰と推定された。
孵化時間は, 水温とは高度に有意な負の相関関係（指数関数的）が認められたが, 塩素量との関係は有意でなかった。水温10-25℃における半数孵化時間D(日）と水温T(℃）の関係は次の3次式で表された。
D=131.3675-19.5853T ＋<special>1.0729T2</special>-<special>0.0202T3</special>},
 pages = {7--13},
 title = {Effects of Temperature and Salinity on Eggs Hatch of the Pond Smelt Hypomesus olidus},
 volume = {1},
 year = {1988}
}