@article{oai:mie-u.repo.nii.ac.jp:00005690,
 author = {嶋田, 協 and Shimada, Kyo and 袴田, 佳宏 and Hakamada, Yoshihiro and 粟冠, 和郎 and Sakka, Kazuo and 松嶋, 欽一 and Matsushima, Kinichi},
 journal = {三重大学生物資源学部紀要 = The bulletin of the Faculty of Bioresources, Mie University},
 month = {Mar},
 note = {application/pdf, Nine strains of cellulolytic-thermophilic anaerobes were isolated from hot springs and composts and
analyzed for their productivity of β-glucosidase (β-D-Glucoside glucohydrolase EC 3. 2. 1. 21). Among them, strain F-9 showed the highest β-glucosidase activity, and this strain was identified as a member of Clostridium thermosaccarolyticum on taxonomical characteristics. The intracellular β-glucosidase of the bacterium was purified 40-fold with a recovery of 6%.
 The purified enzyme preparation was chromatographically homogeneous. The molecular weight of
about 350,000 was estimated by gel filtration using Sephadex G-150, Bio-Gel p-300 and Sepharose 4B.
Its isoelectric point was estimated to be 4.6. The activity of the enzyme for cellobiose was very much
lower than for p-nitrophenyl β-D-glucoside. The enzyme did not hydrolyse maltose, lactose, carboxy-
methyl-cellulose and cellulose. It was strongly inhibited by D-glucono-δ-lactone and was sensitive to
thiol reagents.},
 pages = {81--84},
 title = {堆肥から分離したClostridium thermosaccharolyticum F - 9の β - グルコシダーゼ の性質},
 volume = {2},
 year = {1989}
}