{"created":"2023-06-19T11:38:06.977209+00:00","id":5690,"links":{},"metadata":{"_buckets":{"deposit":"59a733e9-8550-43d3-b635-f1f4b72a68f6"},"_deposit":{"created_by":13,"id":"5690","owners":[13],"pid":{"revision_id":0,"type":"depid","value":"5690"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00005690","sets":["420:421:443:445"]},"author_link":["12325","12326","12327","12328"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1989-03-30","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"84","bibliographicPageStart":"81","bibliographicVolumeNumber":"2","bibliographic_titles":[{"bibliographic_title":"三重大学生物資源学部紀要 = The bulletin of the Faculty of Bioresources, Mie University"}]}]},"item_4_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Nine strains of cellulolytic-thermophilic anaerobes were isolated from hot springs and composts and\nanalyzed for their productivity of β-glucosidase (β-D-Glucoside glucohydrolase EC 3. 2. 1. 21). Among them, strain F-9 showed the highest β-glucosidase activity, and this strain was identified as a member of Clostridium thermosaccarolyticum on taxonomical characteristics. The intracellular β-glucosidase of the bacterium was purified 40-fold with a recovery of 6%.\n The purified enzyme preparation was chromatographically homogeneous. The molecular weight of\nabout 350,000 was estimated by gel filtration using Sephadex G-150, Bio-Gel p-300 and Sepharose 4B.\nIts isoelectric point was estimated to be 4.6. The activity of the enzyme for cellobiose was very much\nlower than for p-nitrophenyl β-D-glucoside. The enzyme did not hydrolyse maltose, lactose, carboxy-\nmethyl-cellulose and cellulose. It was strongly inhibited by D-glucono-δ-lactone and was sensitive to\nthiol reagents.","subitem_description_type":"Abstract"}]},"item_4_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学生物資源学部"}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0915-0471","subitem_source_identifier_type":"PISSN"}]},"item_4_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10073846","subitem_source_identifier_type":"NCID"}]},"item_4_subject_16":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"463","subitem_subject_scheme":"NDC"}]},"item_4_text_18":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Some Properties of β‐Glucosidase from Clostridium thermosaccharolyticum F - 9 Isolated from Compost"}]},"item_4_text_63":{"attribute_name":"ノート","attribute_value_mlt":[{"subitem_text_value":"Agropedia提供データ"}]},"item_4_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper / 紀要論文"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"嶋田, 協","creatorNameLang":"ja"},{"creatorName":"Shimada, Kyo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"袴田, 佳宏","creatorNameLang":"ja"},{"creatorName":"Hakamada, Yoshihiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"粟冠, 和郎","creatorNameLang":"ja"},{"creatorName":"Sakka, Kazuo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"松嶋, 欽一","creatorNameLang":"ja"},{"creatorName":"Matsushima, Kinichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-18"}],"displaytype":"detail","filename":"AN100738460000208.pdf","filesize":[{"value":"249.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN100738460000208.pdf","url":"https://mie-u.repo.nii.ac.jp/record/5690/files/AN100738460000208.pdf"},"version_id":"b49a9c6c-6e49-41d8-aff1-a995357ee345"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"thermophilic","subitem_subject_scheme":"Other"},{"subitem_subject":"β-glucosidase","subitem_subject_scheme":"Other"},{"subitem_subject":"thermostable enzyme","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"堆肥から分離したClostridium thermosaccharolyticum F - 9の β - グルコシダーゼ の性質","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"堆肥から分離したClostridium thermosaccharolyticum F - 9の β - グルコシダーゼ の性質","subitem_title_language":"ja"}]},"item_type_id":"4","owner":"13","path":["445"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-04-26"},"publish_date":"2007-04-26","publish_status":"0","recid":"5690","relation_version_is_last":true,"title":["堆肥から分離したClostridium thermosaccharolyticum F - 9の β - グルコシダーゼ の性質"],"weko_creator_id":"13","weko_shared_id":-1},"updated":"2023-10-12T01:40:32.064875+00:00"}