@article{oai:mie-u.repo.nii.ac.jp:00005698,
 author = {小瀬古, 茂樹 and Koseko, Shigeki and 宮本, 佳典 and Miyamoto, Yoshinori and 久松, 眞 and Hisamatsu, Makoto and 山田, 哲也 and Yamada, Tetsuya},
 journal = {三重大学生物資源学部紀要 = The bulletin of the Faculty of Bioresources, Mie University},
 month = {Feb},
 note = {application/pdf, For improvement of fermentation activity of Zygosaccharomyces rouxii, cell fusion of the yeast with Saccharomyces cerevisiae was attempted by electric field method. The results are following:
  1 For the preparation of protoplast, 2 M KCI and 2 M soribitol is the best to Z, rouxii and 1 M sorbitol is the best to S.cerevisiae as osmotic stabilizer. This observation suggests that favorable osmotic pressure is different to yeast species and mutant.
  2 For the generation of protoplast, 1M KCI is the best and 2 M KCI is better to Z. rouxii as osmotic stabilizer.
  3 The concentration and reaction time of Zymolyase-20 T is proved 1000 ㎍/ml and 60 min as best condition.
  4 Preculture condition of yeast for protoplast preparation was surveyed and 10% NaCl addition to medium is favorable for Z. rouxii (ade-) and no addition is better for Z. rouxii (wild).
  5 A trial of gene transfer of S. cerevisiae to Z. rouxii cell by electroporation method gave six
strains having excellent fermentation activity.},
 pages = {79--85},
 title = {醤油酵母の細胞融合に関する研究 : 第二報　プロトプラスト調整条件の再検討と遺伝子導入法の予備的検討},
 volume = {3},
 year = {1990}
}