@article{oai:mie-u.repo.nii.ac.jp:00005736,
 author = {田口, 寛 and Taguchi, Hiroshi and 若林, 豊 and Wakabayashi, Yutaka and 石原, 則幸 and Ishihara, Noriyuki and 奥村, 克純 and Okumura, Katsuzumi and 嶋林, 幸英 and Shimabayashi, Yoshihide},
 journal = {三重大学生物資源学部紀要 = The bulletin of the Faculty of Bioresources, Mie University},
 month = {Jan},
 note = {application/pdf, A glutamine sensor was constructed by immobilizing L-glutaminase with glutaraldehyde on ISFET
(ion selective field effect transistor) to investigate the possibility of determining L-glutamine. The prin-
ciple of this sensor is as follows. The output voltage from ISFET, which depends on a very minute
pH elevation due to the ammonium ion formation according to the enzymic reaction, is amplified with a
differential amplifier of our own assembled. The amplified voltage was processed automatically with an
on-line personal computer via GP-IB interface on a real-time basis.
 The calibration line for L-glutamine was linear from 1mM to 40mM and the relation coefficient
was 0.967. The sensitivity of this system to L-glutamine was 0.5mM. The time needed for deter-
mining one sample was within 13 min. The substrate specificity of this sensor was strict and L-
glutamine was the sole compound responding to this sensor among analogues tested. D-glutamine was
completely inactive to this sensor.
 It is suggested that L-glutanrine can be determined with this newly constructed ISFET-biosensor
system with L-glutaminase.},
 pages = {71--75},
 title = {ＩＳＦＥＴバイオセンサシステムによるＬ－グルタミン定量の可能性の検討},
 volume = {7},
 year = {1992}
}