{"created":"2023-06-19T11:38:08.952064+00:00","id":5736,"links":{},"metadata":{"_buckets":{"deposit":"caabdfce-252b-40d3-9816-9fbe8e8700b2"},"_deposit":{"created_by":13,"id":"5736","owners":[13],"pid":{"revision_id":0,"type":"depid","value":"5736"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00005736","sets":["420:421:443:450"]},"author_link":["12575","12576","12577","12578","12579"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1992-01-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"75","bibliographicPageStart":"71","bibliographicVolumeNumber":"7","bibliographic_titles":[{"bibliographic_title":"三重大学生物資源学部紀要 = The bulletin of the Faculty of Bioresources, Mie University"}]}]},"item_4_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A glutamine sensor was constructed by immobilizing L-glutaminase with glutaraldehyde on ISFET\n(ion selective field effect transistor) to investigate the possibility of determining L-glutamine. The prin-\nciple of this sensor is as follows. The output voltage from ISFET, which depends on a very minute\npH elevation due to the ammonium ion formation according to the enzymic reaction, is amplified with a\ndifferential amplifier of our own assembled. The amplified voltage was processed automatically with an\non-line personal computer via GP-IB interface on a real-time basis.\n The calibration line for L-glutamine was linear from 1mM to 40mM and the relation coefficient\nwas 0.967. The sensitivity of this system to L-glutamine was 0.5mM. The time needed for deter-\nmining one sample was within 13 min. The substrate specificity of this sensor was strict and L-\nglutamine was the sole compound responding to this sensor among analogues tested. D-glutamine was\ncompletely inactive to this sensor.\n It is suggested that L-glutanrine can be determined with this newly constructed ISFET-biosensor\nsystem with L-glutaminase.","subitem_description_type":"Abstract"}]},"item_4_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学生物資源学部"}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0915-0471","subitem_source_identifier_type":"PISSN"}]},"item_4_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10073846","subitem_source_identifier_type":"NCID"}]},"item_4_subject_16":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"549","subitem_subject_scheme":"NDC"}]},"item_4_text_18":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Investigation of Possibility of Determining L-Glutamine with the ISFET-Biosensor System"}]},"item_4_text_63":{"attribute_name":"ノート","attribute_value_mlt":[{"subitem_text_value":"Agropedia提供データ"}]},"item_4_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper / 紀要論文"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"田口, 寛","creatorNameLang":"ja"},{"creatorName":"Taguchi, Hiroshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"若林, 豊","creatorNameLang":"ja"},{"creatorName":"Wakabayashi, Yutaka","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"石原, 則幸","creatorNameLang":"ja"},{"creatorName":"Ishihara, Noriyuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"奥村, 克純","creatorNameLang":"ja"},{"creatorName":"Okumura, Katsuzumi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"嶋林, 幸英","creatorNameLang":"ja"},{"creatorName":"Shimabayashi, Yoshihide","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-18"}],"displaytype":"detail","filename":"AN100738460000707.pdf","filesize":[{"value":"252.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN100738460000707.pdf","url":"https://mie-u.repo.nii.ac.jp/record/5736/files/AN100738460000707.pdf"},"version_id":"82f6dd83-bd4a-43cc-9830-c9ec3d4471b7"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ISFET","subitem_subject_scheme":"Other"},{"subitem_subject":"biosensor","subitem_subject_scheme":"Other"},{"subitem_subject":"glutamine","subitem_subject_scheme":"Other"},{"subitem_subject":"glutaminase","subitem_subject_scheme":"Other"},{"subitem_subject":"enzyme immobilization","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ＩＳＦＥＴバイオセンサシステムによるＬ－グルタミン定量の可能性の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ＩＳＦＥＴバイオセンサシステムによるＬ－グルタミン定量の可能性の検討","subitem_title_language":"ja"}]},"item_type_id":"4","owner":"13","path":["450"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-05-30"},"publish_date":"2007-05-30","publish_status":"0","recid":"5736","relation_version_is_last":true,"title":["ＩＳＦＥＴバイオセンサシステムによるＬ－グルタミン定量の可能性の検討"],"weko_creator_id":"13","weko_shared_id":-1},"updated":"2023-10-12T02:34:00.407870+00:00"}