{"created":"2023-06-19T11:38:11.467900+00:00","id":5795,"links":{},"metadata":{"_buckets":{"deposit":"a97e8faa-d400-42e9-9318-88404191bcb8"},"_deposit":{"created_by":13,"id":"5795","owners":[13],"pid":{"revision_id":0,"type":"depid","value":"5795"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00005795","sets":["420:421:443:456"]},"author_link":["12853","12854","12855"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1994-12-22","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"6","bibliographicPageStart":"1","bibliographicVolumeNumber":"13","bibliographic_titles":[{"bibliographic_title":"三重大学生物資源学部紀要 = The bulletin of the Faculty of Bioresources, Mie University"}]}]},"item_4_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A simplified and reproducible method in plant breeding using the reverse transcription‐polymerase chain reaction (RT‐PCR) was developed for the selection of plants infected with cucumber mosaic virus (CMV). Total RNA was extracted from CMV-infected leaves of Nicotiana glutinosa by the simplified SDS/phenol method. The RNA was initially reverse-transcribed into cDNA, then the CMV coat protein‐coding sequence was amplified by the PCR method. The detection limit of CMV by the RT‐PCR method ranged from 10 to 100 ng of CMV particles per 10 mg of fresh leaf weight. The sensitivity of the method was similar to that of the ordinary ELISA method.","subitem_description_type":"Abstract"},{"subitem_description":"逆転写-polymerase chain reaction（RT-PCR）法によるキュウリモザイクウイルス（CMV）の遺伝子診断法を開発し\nた。SDS／フェノール法により供試葉から全RNAを抽出し，逆転写酵素により1本鎖cDNAを合成したのち，PCR\nによってCMVの外被タンパク質遺伝子を増幅した。本法によるCMVの検出限界はタバコ葉10mgあたり10～100ngで，ELISA法と同等であった。RNA抽出法の簡便化により1日でCMVの検出が可能になった。本法はCMVの\n新しい診断法としてウイルス抵抗性品種の選抜に用いることができる。","subitem_description_type":"Abstract"}]},"item_4_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Faculty of Bioresources, Mie University"}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0915-0471","subitem_source_identifier_type":"PISSN"}]},"item_4_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10073846","subitem_source_identifier_type":"NCID"}]},"item_4_subject_16":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"624","subitem_subject_scheme":"NDC"}]},"item_4_text_18":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"逆転写-Polymerase Chain Reaction (RT-PCR)によるキュウリモザイクウイルスの検出"}]},"item_4_text_63":{"attribute_name":"ノート","attribute_value_mlt":[{"subitem_text_value":"Agropedia提供データ"}]},"item_4_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper / 紀要論文"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takamatsu, Susumu","creatorNameLang":"en"},{"creatorName":"高松, 進","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"12853","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsuchiya, Takao","creatorNameLang":"en"},{"creatorName":"土屋, 孝夫","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"12854","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Makara, Koichi","creatorNameLang":"en"},{"creatorName":"真柄, 紘一","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"12855","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-18"}],"displaytype":"detail","filename":"AN100738460001301.pdf","filesize":[{"value":"344.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN100738460001301.pdf","url":"https://mie-u.repo.nii.ac.jp/record/5795/files/AN100738460001301.pdf"},"version_id":"8c53531a-6c5c-48be-8afa-b77e615dc7de"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"simplified detection method","subitem_subject_scheme":"Other"},{"subitem_subject":"RT‐PCR","subitem_subject_scheme":"Other"},{"subitem_subject":"cucumber mosaic virus","subitem_subject_scheme":"Other"},{"subitem_subject":"Nicotiana glutinosa","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Detection of Cucumber Mosaic Virus Using the Reverse Transcription-Polymerase Chain Reaction (RT-PCR)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Detection of Cucumber Mosaic Virus Using the Reverse Transcription-Polymerase Chain Reaction (RT-PCR)","subitem_title_language":"en"}]},"item_type_id":"4","owner":"13","path":["456"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-04-23"},"publish_date":"2007-04-23","publish_status":"0","recid":"5795","relation_version_is_last":true,"title":["Detection of Cucumber Mosaic Virus Using the Reverse Transcription-Polymerase Chain Reaction (RT-PCR)"],"weko_creator_id":"13","weko_shared_id":-1},"updated":"2023-10-12T05:55:26.518426+00:00"}