@article{oai:mie-u.repo.nii.ac.jp:00005810,
 author = {Inagaki, Minoru and 稲垣, 穣 and Kato, Muneharu and 加藤, 宗春 and Ohsumi, Yoshiyasu and 大角, 善保 and Kaitani, Koji and 貝谷, 康治 and Nishikawa, Shiro and 西川, 司朗 and Kashimura, Naoki and 柏村, 直樹},
 journal = {三重大学生物資源学部紀要 = The bulletin of the Faculty of Bioresources, Mie University},
 month = {Dec},
 note = {application/pdf, A convenient method for the preparation of a lipopolysaccharide (LPS) with receptor activity of bacteriophage φX174 from large amounts of the dried and defatted cells of Escherichia coli C strain was est.ablished. An improvement on bacterium cultivation led to yield over 75g of the dried and defatted cells (309g wet cells) from 6L culture medium containing 4% wt/v of D-glucose as a carbon source and 1 %wt/v of ammonium sulfate as a nitrogen source. The modified PCP method afforded 2.25g of LPS (3.0% yield from the cells) from 75g of the dried and defatted cells. It was confirmed that the obtained LPS showed in vitro an inactivation of bacteriophage φX174 from a plaque counting assay., バクテリオファージφⅩ174のレセプターとしての生理活性を有するリポ多糖を大腸菌C株の乾燥脱脂薗体から簡
便に大量調製する方法を確立した。培養条件を改良することによって炭素源としてグルコース4％wt／v，窒素源と
して硫安1％wt／vを含む6Lの培養液から75gの乾燥脱脂菌体（湿菌体で309g）を得ることが出来た。得られた菌
体からPCP抽出改良法によってリポ多糖を抽出したところ，2.25gのリポ多糖が得られた（菌体重量の3.0％収率）。
得られたリポ多糖がバクテリオファージφⅩ174のレセプターとしての生理活性を有し，in vitroでのファージ不活
化能力を示すことがプラーク計数法で確認された。},
 pages = {33--40},
 title = {Simple Preparation of Large Amount of Lipopolysaccharide with Receptor Activity for Bacteriophage φXl74 from Escherichia coli C Strain},
 volume = {15},
 year = {1995}
}