{"created":"2023-06-19T11:38:12.108593+00:00","id":5810,"links":{},"metadata":{"_buckets":{"deposit":"64e6348d-092a-41ca-a412-f1419109f96d"},"_deposit":{"created_by":13,"id":"5810","owners":[13],"pid":{"revision_id":0,"type":"depid","value":"5810"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00005810","sets":["420:421:443:458"]},"author_link":["12923","12924","12925","12926","12927","12928"],"control_number":"5810","item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1995-12-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"40","bibliographicPageStart":"33","bibliographicVolumeNumber":"15","bibliographic_titles":[{"bibliographic_title":"三重大学生物資源学部紀要 = The bulletin of the Faculty of Bioresources, Mie University"}]}]},"item_4_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A convenient method for the preparation of a lipopolysaccharide (LPS) with receptor activity of bacteriophage φX174 from large amounts of the dried and defatted cells of Escherichia coli C strain was est.ablished. An improvement on bacterium cultivation led to yield over 75g of the dried and defatted cells (309g wet cells) from 6L culture medium containing 4% wt/v of D-glucose as a carbon source and 1 %wt/v of ammonium sulfate as a nitrogen source. The modified PCP method afforded 2.25g of LPS (3.0% yield from the cells) from 75g of the dried and defatted cells. It was confirmed that the obtained LPS showed in vitro an inactivation of bacteriophage φX174 from a plaque counting assay.","subitem_description_type":"Abstract"},{"subitem_description":"バクテリオファージφⅩ174のレセプターとしての生理活性を有するリポ多糖を大腸菌C株の乾燥脱脂薗体から簡\n便に大量調製する方法を確立した。培養条件を改良することによって炭素源としてグルコース4％wt／v，窒素源と\nして硫安1％wt／vを含む6Lの培養液から75gの乾燥脱脂菌体（湿菌体で309g）を得ることが出来た。得られた菌\n体からPCP抽出改良法によってリポ多糖を抽出したところ，2.25gのリポ多糖が得られた（菌体重量の3.0％収率）。\n得られたリポ多糖がバクテリオファージφⅩ174のレセプターとしての生理活性を有し，in vitroでのファージ不活\n化能力を示すことがプラーク計数法で確認された。","subitem_description_type":"Abstract"}]},"item_4_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Faculty of Bioresources,　Mie University"}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0915-0471","subitem_source_identifier_type":"PISSN"}]},"item_4_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10073846","subitem_source_identifier_type":"NCID"}]},"item_4_subject_16":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"491","subitem_subject_scheme":"NDC"}]},"item_4_text_18":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"バクテリオファージφX１７４レセプター活性を有する大腸菌Ｃ株リポ多糖の簡便大量調製法"}]},"item_4_text_63":{"attribute_name":"ノート","attribute_value_mlt":[{"subitem_text_value":"Agropedia提供データ"}]},"item_4_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper / 紀要論文"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Inagaki, Minoru","creatorNameLang":"en"},{"creatorName":"稲垣, 穣","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kato, Muneharu","creatorNameLang":"en"},{"creatorName":"加藤, 宗春","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ohsumi, Yoshiyasu","creatorNameLang":"en"},{"creatorName":"大角, 善保","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kaitani, Koji","creatorNameLang":"en"},{"creatorName":"貝谷, 康治","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nishikawa, Shiro","creatorNameLang":"en"},{"creatorName":"西川, 司朗","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kashimura, Naoki","creatorNameLang":"en"},{"creatorName":"柏村, 直樹","creatorNameLang":"ja"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-18"}],"displaytype":"detail","filename":"AN100738460001504.pdf","filesize":[{"value":"391.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN100738460001504.pdf","url":"https://mie-u.repo.nii.ac.jp/record/5810/files/AN100738460001504.pdf"},"version_id":"094d7d79-b0a9-4bf7-a2bc-1027155deae0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"LPS","subitem_subject_scheme":"Other"},{"subitem_subject":"E..coli C strain","subitem_subject_scheme":"Other"},{"subitem_subject":"receptor","subitem_subject_scheme":"Other"},{"subitem_subject":"bacteriophage φX174","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Simple Preparation of Large Amount of Lipopolysaccharide with Receptor Activity for Bacteriophage φXl74 from Escherichia coli C Strain","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Simple Preparation of Large Amount of Lipopolysaccharide with Receptor Activity for Bacteriophage φXl74 from Escherichia coli C Strain","subitem_title_language":"en"}]},"item_type_id":"4","owner":"13","path":["458"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-05-10"},"publish_date":"2007-05-10","publish_status":"0","recid":"5810","relation_version_is_last":true,"title":["Simple Preparation of Large Amount of Lipopolysaccharide with Receptor Activity for Bacteriophage φXl74 from Escherichia coli C Strain"],"weko_creator_id":"13","weko_shared_id":-1},"updated":"2023-10-16T01:00:41.489758+00:00"}