@article{oai:mie-u.repo.nii.ac.jp:00005814,
 author = {鄭, 星珠 and Jung, Sung-Ju and 宮崎, 照雄 and Miyazaki, Teruo and 宮田, 雅人 and Miyata, Masato and 大石, 巧 and Oishi, Takumi},
 journal = {三重大学生物資源学部紀要 = The bulletin of the Faculty of Bioresources, Mie University},
 month = {Mar},
 note = {application/pdf, Viral nervous necrosis (VNN) is a disease with Nodavirus among larval and juvenile stages of many species of marine fish. In February 1995, VNN occurred in the juvenile of the Japanese sea bass Lateolabrax japonicus as a new host. The infection of the nodavirus was confirmed by PCR (polymerase chain reaction) method. Histopathological study revealed the brain, spinal cord and retina had nerve cells with coagulative necrosis accompanied by formation of intracytoplasmic PAS positive inclusions and vacuoles. The necrotic cells were replaced by empty spaces after the destruction. In 48-day-old fish, nervous necrosis was marked in the diencephalon and medulla oblongate of the brain, the dorsal part of gray matter in the spinal cord, and the ganglion cell layer and nuclear layer in the retina. In 63-day-old fish, focal necrosis of nerve cells was obvious in all parts of the brain, where a few inflammatory cells followed, and in the dorsal area of the spinal cord. Necrosis of the retinal cells developed with accompanying inflammatory cell infiltration. Electron microscopy revealed uneveloped spherical virus particles (30nm in diameter) present within inclusions or diffusely in the cytoplasm in the brain and spinal cord as well as the retina., 1995年2月，ウイルス性神経壊死症（VNN）が新宿
主であるスズキLateolabrax japonicusに発生し，そ
の48日齢と63日齢の病魚について病理組織学的検討，
電子顕微鏡観察を行った。
病理組織学的に脳，脊髄，網膜の神経細胞は凝固壊死
し，細胞質には空胞が形成されていた。壊死細胞が消失
した跡は円形の空隙として観察された。また，細胞質内
でのPAS反応陽性・ピロエン好性顆粒はウイルス封入
体に起因するものと考えられた。48日齢では脳の間脳
および延髄，脊髄の後柱，網膜の神経細胞層，顆粒層で
壊死が顕著であった。63日齢では巣状壊死が脳の全体
と脊髄の後柱で顕著となり，脳では炎症性細胞浸潤も加
わっていた。網膜細胞の壊死も顕著になり，炎症性細胞
浸潤をともなっていた。
電子顕微鏡観察では脳，脊髄，網膜で，直径30nm
のエンベロープを持たない，円形のウイルス粒子が細胞
質内と封入体内で観察された。},
 pages = {9--16},
 title = {神経壊死症ウイルスの新宿主スズキにおける病理組織像},
 volume = {16},
 year = {1996}
}