{"created":"2023-06-19T11:38:13.189498+00:00","id":5835,"links":{},"metadata":{"_buckets":{"deposit":"da236313-6b65-488a-8e1e-2c14f1ec12ba"},"_deposit":{"created_by":13,"id":"5835","owners":[13],"pid":{"revision_id":0,"type":"depid","value":"5835"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00005835","sets":["420:421:443:463"]},"author_link":["13084","13085","13086","13087"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1998-03-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"45","bibliographicPageStart":"37","bibliographicVolumeNumber":"20","bibliographic_titles":[{"bibliographic_title":"三重大学生物資源学部紀要 = The bulletin of the Faculty of Bioresources, Mie University"}]}]},"item_4_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In cells undergoing apoptosis, the characteristic \"ladder\" pattern of DNA fragments is observed on agarose gel electrophoresis. Such a pattern of DNA degradation generally serves as a criterion for the apoptosis. However, on agarose gel electrophoresis it is difficult to accurately quantify DNA fragmentation. Therefore, quantification of DNA fragmentation is determined by flow cytometry, DNA fragmentation assay etc. In this paper we improved the methods for the analysis of apoptosis by flow cytometry. As\na result, we can obtain much information (quantification of apoptosis and cell cycle analysis etc.) from a single sample (1~3×10 6 cells) with the improved methods. The sample is treated with phosphate-citrate buffer. These methods are very simple and\nconvenient.","subitem_description_type":"Abstract"}]},"item_4_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学生物資源学部"}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0915-0471","subitem_source_identifier_type":"PISSN"}]},"item_4_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN10073846","subitem_source_identifier_type":"NCID"}]},"item_4_subject_16":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"463","subitem_subject_scheme":"NDC"}]},"item_4_text_18":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Improved methods for analysis of apoptosis by flow cytometry"}]},"item_4_text_65":{"attribute_name":"資源タイプ(三重大)","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper / 紀要論文"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"緒方, 進","creatorNameLang":"ja"},{"creatorName":"Ogata, Shin","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"武内, 昌代","creatorNameLang":"ja"},{"creatorName":"Takeuchi, Masayo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"奥村, 克純","creatorNameLang":"ja"},{"creatorName":"Okumura, Katsuzumi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"田口, 寛","creatorNameLang":"ja"},{"creatorName":"Taguchi, Hiroshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-18"}],"displaytype":"detail","filename":"50C12501.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"50C12501.pdf","url":"https://mie-u.repo.nii.ac.jp/record/5835/files/50C12501.pdf"},"version_id":"410f361a-811a-4df4-af5d-a7936b15db46"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"flow cytometry","subitem_subject_scheme":"Other"},{"subitem_subject":"apoptosis","subitem_subject_scheme":"Other"},{"subitem_subject":"cell cycle","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA fragmentation","subitem_subject_scheme":"Other"},{"subitem_subject":"hydrogen peroxide","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"フローサイトメトリーによるアポトーシス解析の改良法","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"フローサイトメトリーによるアポトーシス解析の改良法","subitem_title_language":"ja"}]},"item_type_id":"4","owner":"13","path":["463"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2010-03-17"},"publish_date":"2010-03-17","publish_status":"0","recid":"5835","relation_version_is_last":true,"title":["フローサイトメトリーによるアポトーシス解析の改良法"],"weko_creator_id":"13","weko_shared_id":-1},"updated":"2023-10-12T07:16:51.661386+00:00"}