@article{oai:mie-u.repo.nii.ac.jp:00005836,
 author = {Okumura, Katsuzumi and 奥村, 克純 and Kagotani, Kazuhiro and 籠谷, 和弘 and Kohda, Atsushi and 香田, 淳 and Nogami, Masahiro and 野上, 正弘 and Hishida, Koji and 菱田, 浩司 and Taguchi, Hiroshi and 田口, 寛},
 journal = {三重大学生物資源学部紀要 = The bulletin of the Faculty of Bioresources, Mie University},
 month = {Mar},
 note = {application/pdf, We have analyzed the replication timing of DNA segments of the imprinted IGF2
(insulin-like growth factor II ) gene and non-imprinted genes in human lymphocytes by fluorescence in situ hybridization (FISH). In principle, intranuclear FISH signals of each DNA segment are characterized in three groups according to the replication status of each parental allele; two singlets (SS), singlet-doublet (SD), and two doublets (DD). Non-imprinted genes, both of ?NF (tumor necrosis factor) and RFC5 (replication factor C subunit 5), showed a smaller percentage of the SD pattern, while IGF2 gene had a higher percentage of the SD pattern, suggesting that the imprinted IGF2 gene replicates asynchronously between its homologous alleles. As the results, together with previous observation, we concluded the temporal order of DNA replication of three genes in human lymphocytes to be as follows : Both alleles of TNF, paternal allele of 1GF2, both alleles of RFC5, and then maternal allele of IGF2., 蛍光 in situ hybridization (FISH)法を用いて, ヒト0リンパ球におけるDNA複製タイミングをゲノム刷り込みを受けるIGF2(インスリン様成長因子型)と受けないTNF(がん壊死因子)およびRFC5(複製因子C第5サブユニット)遺伝子について解析した。各ゲノム断片の細胞核内FISHシグナルは,原理的に,それぞれが由来する親アレルの複製状態に従って,2つのシングルドット(SS),シングルドットとダブルドット各一個(SD),2つのダブルドット(DD)の3つのグループに大別される。ゲノム刷り込みを受けないTNF, RFC5は, SDパターンの割合が小さいが,刷り込みを受けるIGF2はSDパターンが高く,アレル間で非同調的に複製することが示唆された。これらの結果とこれまでの報告とあわせて, これらの遺伝子の複製が,TNFの両アレル,IGF2の父方アレル,RFC5の両アレル,IGF2の母方アレルの順に進行すると結論した。},
 pages = {47--54},
 title = {Temporal order of DNA replication of imprinted IGF2 gene and Non-imprinted TNF and RFC5 genes in human lymphocytes},
 volume = {20},
 year = {1998}
}