{"created":"2023-06-19T11:38:28.096723+00:00","id":6159,"links":{},"metadata":{"_buckets":{"deposit":"05e0babe-99ad-4415-a533-6412658037b1"},"_deposit":{"created_by":13,"id":"6159","owners":[13],"pid":{"revision_id":0,"type":"depid","value":"6159"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00006159","sets":["420:421:481:485"]},"author_link":["13971","13972","13973"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-02-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"97","bibliographicPageStart":"87","bibliographicVolumeNumber":"37","bibliographic_titles":[{"bibliographic_title":"三重大学大学院生物資源学研究科紀要"}]}]},"item_4_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Muscle tissues are generally classified into three types, skeletal, cardiac, and smooth muscle. Smooth muscle cells (SMCs) include all muscle cells other than skeletal and cardiac muscles. There are various types of smooth muscle tissues, and they play important roles in many physiological functions. SMCs are localized in digestive tract (such as esophagus, stomach, ileum, small intestine, large intestine), blood vessels, uterus, airway, and bladder. Response to agonists of these SMCs varied from one muscle type to other type. The main mechanism of activation of contraction is transient increment of cytoplasmic concentration of Ca2+, followed by phosphorylation of myosin light chain. Some actin-binding proteins activate or inhibit the above actomyosin-based contraction. This regulatory mechanism of the contraction is thought to be universal among various SMCs. Since SMCs of these tissues are small and furthermore surrounded by large amount of extra cellular matrix or mucous membrane, we can isolate only a small amount of SMCs purely. For biochemical study of muscle proteins, selected SMCs, such as avian gizzard, bovine stomach, bovine aorta, are usually used for convenient starting materials. Most of genes of major muscle proteins in SMCs are isolated so far. Therefore, these genes can be expressed in bacterial, insect, or cultured animal cells. However, myosin or other muscle proteins have been purified from animal tissues still now, because of complexity of construction of expression vectors or economical reasons of these expression systems. In this report we describe improved and convenient methods to prepare myosin and related regulatory proteins.","subitem_description_type":"Abstract"}]},"item_4_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学大学院生物資源学研究科"}]},"item_4_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12234310","subitem_source_identifier_type":"NCID"}]},"item_4_text_18":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Improved methods for preparation of myosin and its related regulatory proteins from smooth muscle tissue"}]},"item_4_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper / 紀要論文"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"近藤, 哲也","creatorNameLang":"ja"},{"creatorName":"Kondo, Tetsuya","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"鈴木, 亮","creatorNameLang":"ja"},{"creatorName":"Suzuki, Ryo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"岡垣, 壮","creatorNameLang":"ja"},{"creatorName":"Okagaki, Tsuyoshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-18"}],"displaytype":"detail","filename":"50C14412.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"50C14412.pdf","url":"https://mie-u.repo.nii.ac.jp/record/6159/files/50C14412.pdf"},"version_id":"d3eda232-7b1b-46bb-bb57-e78674ec381a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"smooth muscle","subitem_subject_scheme":"Other"},{"subitem_subject":"myosin","subitem_subject_scheme":"Other"},{"subitem_subject":"MLCK","subitem_subject_scheme":"Other"},{"subitem_subject":"caldesmon","subitem_subject_scheme":"Other"},{"subitem_subject":"telokin","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"平滑筋からの myosin および調節タンパク質の精製法の改良","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"平滑筋からの myosin および調節タンパク質の精製法の改良","subitem_title_language":"ja"}]},"item_type_id":"4","owner":"13","path":["485"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2011-02-17"},"publish_date":"2011-02-17","publish_status":"0","recid":"6159","relation_version_is_last":true,"title":["平滑筋からの myosin および調節タンパク質の精製法の改良"],"weko_creator_id":"13","weko_shared_id":-1},"updated":"2023-10-10T05:16:51.210358+00:00"}