{"created":"2023-06-19T11:38:30.592367+00:00","id":6198,"links":{},"metadata":{"_buckets":{"deposit":"17842e8c-cd31-48ec-a439-49749e05b615"},"_deposit":{"created_by":13,"id":"6198","owners":[13],"pid":{"revision_id":0,"type":"depid","value":"6198"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00006198","sets":["420:421:481:487"]},"author_link":["14019","14020","14021"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"60","bibliographicPageStart":"55","bibliographicVolumeNumber":"39","bibliographic_titles":[{"bibliographic_title":"三重大学大学院生物資源学研究科紀要"}]}]},"item_4_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Laminarioligosaccharides (LOS) are used as substrates or inhibitors of several carbohydrate-active\nenzymes, and exhibit several bioactivities. Commercial LOS are very expensive. In this study, we report\na simple method for preparing high-purity laminaribiose (L2) and laminaritriose (L3) from curdlan,\nwhich is a linear, high-molecular-weight β-1, 3-glucan that is available at low cost. Crude LOS were\nobtained from the degradation of curdlan by using a commercial enzyme, Zymolyase-20T, prepared from\na submerged culture of Arthrobacter luteus. Thin-layer chromatography (TLC) analysis showed that the\nmain components of crude LOS were glucose, L2, and L3. In addition, slight amounts of LOS larger than \nL3 were observed. L2 and L3 were purified from crude LOS by activated carbon　chromatography, and\ntheir purities were confirmed by TLC and high-performance liquid chromatography (HPLC). Finally, 1.7\ng of L2 and 1.2 g of L3 were obtained from 10g of curdlan.","subitem_description_type":"Abstract"}]},"item_4_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学大学院生物資源学研究科"}]},"item_4_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA12234310","subitem_source_identifier_type":"NCID"}]},"item_4_text_18":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"カードランの酵素加水分解によるラミナリビオースとラミナリトリオースの簡易調製"}]},"item_4_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper / 紀要論文"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Naoto, ISONO","creatorNameLang":"en"},{"creatorName":"磯野, 直人","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"14019","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tomomi, KIMOTO","creatorNameLang":"en"},{"creatorName":"木本, 智美","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"14020","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Makoto, HISAMATSU","creatorNameLang":"en"},{"creatorName":"久松, 眞","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"14021","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-18"}],"displaytype":"detail","filename":"50C16194.pdf","filesize":[{"value":"587.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"50C16194.pdf","url":"https://mie-u.repo.nii.ac.jp/record/6198/files/50C16194.pdf"},"version_id":"b2a9506b-5eea-4c8c-a2de-1a5c2d02d327"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"laminaribiose","subitem_subject_scheme":"Other"},{"subitem_subject":"laminaritriose","subitem_subject_scheme":"Other"},{"subitem_subject":"laminarioligosaccharides","subitem_subject_scheme":"Other"},{"subitem_subject":"curdlan","subitem_subject_scheme":"Other"},{"subitem_subject":"activated carbon","subitem_subject_scheme":"Other"},{"subitem_subject":"chromatography","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A Simple Method for Preparing Laminaribiose and Laminaritriose from the Enzymatic Hydrolysates of Curdlan","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A Simple Method for Preparing Laminaribiose and Laminaritriose from the Enzymatic Hydrolysates of Curdlan","subitem_title_language":"en"}]},"item_type_id":"4","owner":"13","path":["487"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2013-04-11"},"publish_date":"2013-04-11","publish_status":"0","recid":"6198","relation_version_is_last":true,"title":["A Simple Method for Preparing Laminaribiose and Laminaritriose from the Enzymatic Hydrolysates of Curdlan"],"weko_creator_id":"13","weko_shared_id":-1},"updated":"2023-10-10T06:07:53.296825+00:00"}