{"created":"2023-06-19T11:38:37.416362+00:00","id":6357,"links":{},"metadata":{"_buckets":{"deposit":"8f3124da-52a8-4a18-b65b-ae55c2efd66f"},"_deposit":{"created_by":13,"id":"6357","owners":[13],"pid":{"revision_id":0,"type":"depid","value":"6357"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00006357","sets":["420:421:498:505"]},"author_link":["14616","14617","14618","14619","14620"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1983-12-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"113","bibliographicPageStart":"107","bibliographicVolumeNumber":"67","bibliographic_titles":[{"bibliographic_title":"三重大學農學部學術報告 = The bulletin of the Faculty of Agriculture, Mie University"}]}]},"item_4_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"還元性糖類のウイルス不活化作用の機構を解明するため，D-リボースによる，タバコモザイクウイルス(TMV）とバクテリオファージØX174の in vitro 不活化およびニトロブルーテトラゾリウム（NBT）の還元が研究された。TMVとØX174は，10⁻⁴MのCu²⁺の存在下，D-リボースによって効率よく不活化された。しかし，他の金属イオン，例えばFe³⁺，Mn²⁺，Zn²⁺は不活化を促進しなかった。\n不活化反応は，カタラーゼまたはチロンで阻啓されたが，このことは，不活化が，D-リポースの自動酸化で生成した酸素ラジカルによってウイルス核酸の生物活性が失われたためであることを強く示唆した。NBT還元反応がスーパーオキシドジスムターゼによって阻害されることでわかるように，D-リボースは，スーパーオキシドを生成することが見出された。また，D-リボースのNBT還元速度はD-リポース5-リン酸エステルやピラノシドゥロースの速度よりもかなり遅いことがわかった。本実験で得られたデーターとこれまでの研究で得られた結果から，本ウイルス不活化作用の可能なメカニズム(複数)が推察されたが，そのなかで，D-リボースの自動酸化におけるCu²⁺の関与および酸素の一電子還元においてエンジオールの中間体として寄与が考察された。","subitem_description_type":"Abstract"},{"subitem_description":"In vitro inactivation of tobacco mosaic virus (TMV) and bacteriophage ØX174, and reduction of Nitro Blue tetrazolium chloride (NBT), by D-ribose were studied for the elucidation of the reaction mechanism of virus inactivation by reducing sugars. TMV and ØX174 were effetctively inactivated by D-ribose in the presence of Cu²⁺(10⁻⁴M), but other metal ions such as Fe³⁺, Mn²⁺, Zn²⁺ did not stimulate the inactivation. \nThe inactivation reactions were inhibited by catalase or tiron. This fact strongly suggested that the inactivation is due to the loss of biological activity of the virus nucleic acids caused by oxygen radicals generated by the autoxidation of D-ribose. It was found that D-ribose generates superoxide  as revealed by inhibition by superoxide dismutase of NBT reduction, and that the rate of NBT reduction is much slower than those of D-ribose 5-phosphate and pyranosiduloses. \nFrom the present data, together with previously obtained results, possible mechanisms for the virus inactivations were proposed in which contribution of cupric ion to the autoxidation of D-ribose and the intermediacy of endiolate anion in univalent reduction of oxygen by D-ribose were discussed.","subitem_description_type":"Abstract"}]},"item_4_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学農学部"}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0462-4408","subitem_source_identifier_type":"PISSN"}]},"item_4_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00234337","subitem_source_identifier_type":"NCID"}]},"item_4_text_18":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"On in vitro Inactivation of Tobacco Mosaic Virus and Bacteriophage ØX174 by D－Ribose"}]},"item_4_text_63":{"attribute_name":"ノート","attribute_value_mlt":[{"subitem_text_value":"Agropedia提供データ"}]},"item_4_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper / 紀要論文"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"柏村, 直樹","creatorNameLang":"ja"},{"creatorName":"Kashimura, Naoki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"佐藤, 郁夫","creatorNameLang":"ja"},{"creatorName":"Sato, Ikuo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"熊澤, 善三郎","creatorNameLang":"ja"},{"creatorName":"Kumazawa, Zenzaburo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"小山, 司朗","creatorNameLang":"ja"},{"creatorName":"Koyama, Shiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"酒井, 昭弘","creatorNameLang":"ja"},{"creatorName":"Sakai, Akihiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-18"}],"displaytype":"detail","filename":"AN002343370006709.pdf","filesize":[{"value":"436.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN002343370006709.pdf","url":"https://mie-u.repo.nii.ac.jp/record/6357/files/AN002343370006709.pdf"},"version_id":"5ac296b0-1b7c-46c4-a831-f387f8934e39"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"D-リボースによるタバコモザイクウイルスおよびバクテリオファージØX174の in vitro 不活化反応について","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"D-リボースによるタバコモザイクウイルスおよびバクテリオファージØX174の in vitro 不活化反応について","subitem_title_language":"ja"}]},"item_type_id":"4","owner":"13","path":["505"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-07-05"},"publish_date":"2007-07-05","publish_status":"0","recid":"6357","relation_version_is_last":true,"title":["D-リボースによるタバコモザイクウイルスおよびバクテリオファージØX174の in vitro 不活化反応について"],"weko_creator_id":"13","weko_shared_id":-1},"updated":"2023-10-13T05:03:56.669297+00:00"}