{"created":"2023-06-19T11:38:38.311984+00:00","id":6378,"links":{},"metadata":{"_buckets":{"deposit":"d38c1563-8444-436f-b576-b1067ff3be2c"},"_deposit":{"created_by":13,"id":"6378","owners":[13],"pid":{"revision_id":0,"type":"depid","value":"6378"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00006378","sets":["420:421:498:509"]},"author_link":["14720","14721"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1985-12-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"12","bibliographicPageStart":"1","bibliographicVolumeNumber":"71","bibliographic_titles":[{"bibliographic_title":"三重大學農學部學術報告 = The bulletin of the Faculty of Agriculture, Mie University"}]}]},"item_4_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To examine the changes in physiological activity and radiosensitivity of cultured cells during a culture period, the long‐term subcultured callus cells of the rice viriety \"Sensho\" which refer to as SC‐cell line were used as experimental materials. The SC‐cell was characterized  by considerably homogeneous diploid cell population and by sigmoidal growth pattern with a long doubling time (78.7 hr) in log phase. \nIn the SC‐cell line, the mid log phase was the most sensitive to X‐rays, the stationary phase the most resistant and the lag phase intermediate. Differential radiosensitivities in various growth phases coincided with change in the frequency of ³H-thymidine labeled cells, indicating that the higher frequency of DNA-synthesizing cells correlates with the higher radiosensitivity. Respiration activity measured by O₂ uptake did not directly affect the radiosensitivity. The cultured cells at stationary phase exhibited the same degree of radiosensitivity as the seedlings. The present results suggest that cytological factors such as the frequency of DNA‐synthesizing cells and mitotic activity take part in determination of the radiosensitivities at different growth phases of cultured cells in higher plants.","subitem_description_type":"Abstract"},{"subitem_description":"イネ品種\"戦捷\"の根端から誘導し2年以上にわたって長期間液体培養をしたカルス細胞系統（SC-細胞系統）を用いて，培養期間中の種々の生育相における放射線感受性と細胞学的・生理学的活性の変化を調査した。供試したSC-細胞系統は比較的均一な2倍体細胞集団から成り，25回以上の継代培養においても旺盛な増殖を示す細胞系統であり，対数増殖期における増殖量倍化時間は78.7時間であった。\nこのSC-細胞系統において，2.5～10kR のX線を照射した後，細胞増殖量によって放射線感受性を調べた結果，対数増殖中期の感受性が最も高く(D50=2.2kR) 定常期は最も抵抗性であって(D50=6.9kR)，約3倍の感受性の差異が認められた。さらに様々の生育相において，³H-チミジンの細胞核内取り込み頻度をミクロオートラジオグラフイー法によって調査し，DNA合成期細胞の頻度を推定した。またワールブルグ法により各生育相の呼吸活性を調べた。その結果，DNA合成期細胞の頻度が高い生育相ほど放射線感受性が高いこと，および呼吸活性と感受性との間には直接的な関係は認められないことが明らかとなった。以上のことから，高等植物の培養細胞において細胞核のDNA合成や細胞分裂などの細胞学杓活性が放射線感受性の主な支配要因として作用していると考えられる。","subitem_description_type":"Abstract"}]},"item_4_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学農学部"}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0462-4408","subitem_source_identifier_type":"PISSN"}]},"item_4_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00234337","subitem_source_identifier_type":"NCID"}]},"item_4_text_18":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"イネ培養細胞の各生育相における放射線感受性と細胞学的活性との関係"}]},"item_4_text_63":{"attribute_name":"ノート","attribute_value_mlt":[{"subitem_text_value":"Agropedia提供データ"}]},"item_4_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper / 紀要論文"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kowyama, Yasuo","creatorNameLang":"en"},{"creatorName":"神山, 康夫","creatorNameLang":"ja"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Shibata, Norio","creatorNameLang":"en"},{"creatorName":"柴田, 憲男","creatorNameLang":"ja"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-18"}],"displaytype":"detail","filename":"AN002343370007101.pdf","filesize":[{"value":"758.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN002343370007101.pdf","url":"https://mie-u.repo.nii.ac.jp/record/6378/files/AN002343370007101.pdf"},"version_id":"9fd17523-5dc5-4070-b6cc-2106844a9a55"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Radiosensitivity and Cytological Activity at Different Growth Phases of Rice Cells in Suspension Culture","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Radiosensitivity and Cytological Activity at Different Growth Phases of Rice Cells in Suspension Culture","subitem_title_language":"en"}]},"item_type_id":"4","owner":"13","path":["509"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-07-10"},"publish_date":"2007-07-10","publish_status":"0","recid":"6378","relation_version_is_last":true,"title":["Radiosensitivity and Cytological Activity at Different Growth Phases of Rice Cells in Suspension Culture"],"weko_creator_id":"13","weko_shared_id":-1},"updated":"2023-10-13T05:43:32.442602+00:00"}