{"created":"2023-06-19T11:38:38.701946+00:00","id":6387,"links":{},"metadata":{"_buckets":{"deposit":"e1a35290-1b26-46fc-a2b8-b140c9a77d7f"},"_deposit":{"created_by":13,"id":"6387","owners":[13],"pid":{"revision_id":0,"type":"depid","value":"6387"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00006387","sets":["420:421:498:509"]},"author_link":["14764","14765","14766","14767"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1985-12-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"104","bibliographicPageStart":"97","bibliographicVolumeNumber":"71","bibliographic_titles":[{"bibliographic_title":"三重大學農學部學術報告 = The bulletin of the Faculty of Agriculture, Mie University"}]}]},"item_4_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"先の報告で，菌体外に蛋白質を生産する酵母の選択を行い，キシロース資化性菌として著者の一人赤木が分離した X-19 号菌を選択し，その菌学的性質を調べ同定を行い，Trichosporon属の新種であることを報告した。\n本報告では，選択されたTrichosporon sp. X-19 株の大量培養について検討した。\nCzapek－Dox 改良培地がこの酵母のECP 生産に有用であり，培地の窒素源として試験した20種類のアミノ酸の中，グリシン，アルギニン，アスパラギンがECP生産に良好であった。アルギニンを窒素源とした場合は，中性に近い培養でもECPを生産した。\n30l 容ジャーファメンターを用いて，3種類の窒素源についてECP生産の比較を行った。グリシンはアルギニンとほぼ同量のECPを生産したが，燐酸アンモンでは約1/2の収量であった。\n増殖用タンクの全容数 800l の中間工業試験用培養装置を用いて，運転容量 500l で大数培養を2回行い，1.3 KgのECPと 1.9 Kg の乾燥菌体を得た。\nECPの栄養価については，他の報告で述べた。","subitem_description_type":"Abstract"},{"subitem_description":"Ｉn the previous papaers, we have reported on the selection of yeast strain producing extracellular protein(ECP), and morphological and physiological characteristics of the selected strain. The selected yeast, X-19 strain, which had been isolated as a xylose utilizer by M. Akaki, one of the authors, produced appreciable amount of protein in YM medium, and this strain proved to be a new species belonging to the genus Trichosporon. \nIn this paper, we have discussed large scale production of ECP by the selecled strain, Trichosporon sp. X-19. Instead of YM midium, Czapek-Dox medium modified (glycine 0.3%, K₂HPO₄ 0.1%, MgSO₄7H₂O 0.05%, KCl 0.05%, FeSO₄・7H₂O 0.001%, glucose 3.0%) proved to be useful for ECP production. Among tewnty kinds of amino acid used as nitrogen source in the culture medium, glycine, arginine and asparagine gave good ECP producttion. Also it was shown that arginine has special properties to allow production of ECP by the yeast even at neutral conditions of the culture. \nComparison of ECP production by yeast, using jar fermentor with three kinds of nitrogen source, i. e., glycine，arginine and ammonium phosphate monobasic, was made. The full capaciiy of the fermentor used was 30 liters, and normal operating volume was 20 liters. As a result, glycine gave nearly the same ECP yield as arginine, and ammonium phosphate monobasic gave half the same ECP yield. \nLarge scale cultivation with this yeast was carried out twice by using pilot plant cultivation equipment, and 1.3 Kg of ECP and 1.9 Kg of dry cell were recovered. The full capacity of progagation tank was 800 liters and normal working volume was 500 liters. \nNutritional evaluation of the ECP was discussed in the other papers.","subitem_description_type":"Abstract"}]},"item_4_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学農学部"}]},"item_4_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0462-4408","subitem_source_identifier_type":"PISSN"}]},"item_4_source_id_9":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00234337","subitem_source_identifier_type":"NCID"}]},"item_4_text_18":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Studies on Extracellular Protein Production by Yeast Cultural Conditions for Mass Cultivation of Trichosporon sp. X-19"}]},"item_4_text_63":{"attribute_name":"ノート","attribute_value_mlt":[{"subitem_text_value":"Agropedia提供データ"}]},"item_4_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper / 紀要論文"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"赤木, 盛郎","creatorNameLang":"ja"},{"creatorName":"Akaki, Morio","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"西田, 淑男","creatorNameLang":"ja"},{"creatorName":"Nishida, Yoshio","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"久松, 眞","creatorNameLang":"ja"},{"creatorName":"Hisamatsu, Makoto","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"山田, 哲也","creatorNameLang":"ja"},{"creatorName":"Yamada, Tetsuya","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-18"}],"displaytype":"detail","filename":"AN002343370007110.pdf","filesize":[{"value":"441.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN002343370007110.pdf","url":"https://mie-u.repo.nii.ac.jp/record/6387/files/AN002343370007110.pdf"},"version_id":"c9ba8c67-1237-4b27-96af-d0daf8317554"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"酵母による菌体外蛋白質生産に関する研究 : Trichosporon sp. X-19 の大量培養","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"酵母による菌体外蛋白質生産に関する研究 : Trichosporon sp. X-19 の大量培養","subitem_title_language":"ja"}]},"item_type_id":"4","owner":"13","path":["509"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-07-20"},"publish_date":"2007-07-20","publish_status":"0","recid":"6387","relation_version_is_last":true,"title":["酵母による菌体外蛋白質生産に関する研究 : Trichosporon sp. X-19 の大量培養"],"weko_creator_id":"13","weko_shared_id":-1},"updated":"2023-10-13T05:43:58.122636+00:00"}