{"created":"2023-06-19T11:38:40.538573+00:00","id":6430,"links":{},"metadata":{"_buckets":{"deposit":"08cd3b19-5bb9-4cd9-ba52-5feef571ca9c"},"_deposit":{"created_by":13,"id":"6430","owners":[13],"pid":{"revision_id":0,"type":"depid","value":"6430"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00006430","sets":["515:516:517:518"]},"author_link":["14955","14956","14957","14958"],"item_4_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-10-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"16","bibliographicPageStart":"13","bibliographic_titles":[{"bibliographic_title":"Proceedings of the Second International Workshop on Regional Innovation Studies :  (IWRIS2010)"}]}]},"item_4_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_4_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Rhizoctonia spp. consists of genetically\nincompatible strain groups (anastomosis group, AG)\ndistinguished by lack of anastomosis between hyphae of strains.\nIn recent years, nucleotide sequences of rDNA-ITS region are\nwidely used for the analysis of molecular diagnoses,\nclassifications of DNA barcode and evolutions of plant\npathogenic fungi. In this study, six different taxa of genus\nRhizoctonia, includes three genus Thanatephorus, Waitea and\nCeratobasidium on the teleomorph, of rice and weed sclerotial\nfungi, were classified with PCR-RFLP band patterns. Eight\nisolates of bincleate Rhizoctonia AG-Ba, the gray sclerotial\nfungus, showed three banding patterns, Type I (4 isolates),\nType II (2 isolates) and a mixed-banding type (2 isolates). To\ndetermine whether the mixed-banding type arose from genome\nmixing between Type I and II, we cloned PCR products of ITS\nregions include 5.8S rDNA sequence. Twelve sequences\nderived from each isolates were sequenced from both\ndirections and multiply aligned. The cluster analysis based on\nthe multiple alignment showed that sequences derived from\neach isolate divided into two clades. The sequences derived\nfrom mixed-banding type isolates were divided into both two\nclades, revealing these isolates caused from genome crossing\nbetween Type I and II. To confirm this interpretation,\nmicrosatellite fingerprints of 54 isolates of binucleate\nRhizoctonia AG-Ba were investigated and the fingerprint\npatterns were analyzed with cluster analysis. Unexpectedly,\nfingerprint patterns derived from 54 isolates were divided into\nthree clades, unlike results of ITS sequencing. The result of\ncluster analysis likely to cause by unique banding patterns of\nthe mixed type isolates, although banding patterns of few\nisolates showed mixed character of Type I and II. Because of\nsuperior information of microsatellite fingerprinting, the\nresults lead us to interpretation that Rhizoctonia AG-Ba\nisolates should be classified into at least three subgroups.","subitem_description_type":"Abstract"}]},"item_4_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Graduate School of Regional Innovation Studies, Mie University"}]},"item_4_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Departmental Bulletin Paper / 紀要論文"}]},"item_4_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Urushizaki, Shingo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"14955","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Inagaki, Kimiharu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"14956","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kobayashi, Issei","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"14957","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Arakawa, Masao","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"14958","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-18"}],"displaytype":"detail","filename":"60C15225.pdf","filesize":[{"value":"150.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"60C15225.pdf","url":"https://mie-u.repo.nii.ac.jp/record/6430/files/60C15225.pdf"},"version_id":"6eee9ca9-8e2c-4a9e-93db-41450816ed33"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Rhizoctonia AG-Ba","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"rDNA-ITS region","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"RFLP analysis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Sequence analysis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA Fingerprinting","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Molecular Characterization of the Subgroup Differentiation in Binucleate Rhizoctonia AG-Ba, causal agents of rice sclerotial diseases.","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Molecular Characterization of the Subgroup Differentiation in Binucleate Rhizoctonia AG-Ba, causal agents of rice sclerotial diseases.","subitem_title_language":"en"}]},"item_type_id":"4","owner":"13","path":["518"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2011-11-07"},"publish_date":"2011-11-07","publish_status":"0","recid":"6430","relation_version_is_last":true,"title":["Molecular Characterization of the Subgroup Differentiation in Binucleate Rhizoctonia AG-Ba, causal agents of rice sclerotial diseases."],"weko_creator_id":"13","weko_shared_id":-1},"updated":"2023-09-19T06:23:31.043928+00:00"}