{"created":"2023-06-19T11:39:24.535869+00:00","id":7448,"links":{},"metadata":{"_buckets":{"deposit":"3685294c-3545-4bdd-ad6c-89cfd728f921"},"_deposit":{"created_by":15,"id":"7448","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"7448"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00007448","sets":["334:606:607"]},"author_link":[],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1983-08-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"868","bibliographicPageStart":"861","bibliographicVolumeNumber":"26","bibliographic_titles":[{"bibliographic_title":"糖尿病"}]}]},"item_10001_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"\"妊娠時の糖代謝の特徴の１つとして,インスリンに対する抵抗性の増大があげられるが,その機序を解明する目的で,妊娠後期ラットより調製した単離脂肪細胞を用いて,インスリンのグルコース酸化促進作用およびインスリン受容体について検討した. インスリンのグルコース酸化促進作用は,37℃ 60分間に(1-¹⁴C)グルコースより発生する¹⁴CO₂の量を,インスリン受容体は,37℃ 60分間または24℃ 90分間に結合する¹²⁵I-インスリン量を,それぞれ各種濃度のインスリン存在下で測定することにより求めた. 単位脂肪細胞数あたりのグルコース酸化作用は,インスリン非存在下および存在下とも妊娠ラットのほうが非妊娠ラットに比し有意に低下していた. ¹²⁵I-インスリン結合率については37℃および24℃の両条件下とも各種濃度のインスリン存在下で両ラット間に有意差は認められなかった. 結合率測定中のインスリンの分解率についても両ラット間で有意差は認められなかった.\n以上の成績により,妊娠後期ラットの単離脂肪細胞ではグルコース酸化作用に関してインスリン抵抗性が認められるが,それは主としてインスリンに対する responsiveness の低下によるものであり,しかも,インスリン受容体の数および親和性に有意の変化が認められず,妊娠時のインスリン抵抗性がインスリン受容体以後(postreceptor)の過程の変化により生じていることが示唆された.","subitem_description_type":"Abstract"},{"subitem_description":"Increased insulin resistance is one of the characteristics of metabolism during pregnancy. We studied the action of insulin on glucose oxidation and the insulin receptors using isolated adipocytes prepared from late pregnant rats in order to clarify the mechanism of the insulin resistance. The insulin action on glucose oxidation was estimated by measuring the amount of ¹⁴CO₂ converted by adipocytes from (1-¹⁴C) glucose during 60 min at 37℃. The insulin receptors were assessed by measuring the ¹²⁵I-insulin bound to the adipocytes during 60 min at 37℃ or during 90 min at 24℃. The glucose oxidation was significantly decreased in the pregnant rat adipocytes with or without insulin. The amount of ¹⁴CO₂ converted from (1-¹⁴C) glucosc by adipocytes from pregnant and nonpregnant rats was 2.81±0.49 and 4.62±0.54 without insulin, and 16.04±2.10 and 26. 1±3.43 (n=7, mean±SEM) with 25 ng/ml insulin, respectively. There was no significant difference in the ¹²⁵I-insulin binding at each insulin concentration between the pregnant and nonpregnant rats. There was also no significant difference in the degradation of ¹²⁵I-insulin during the incubation time.\nThere results suggest that isolated adipocytes from late pregnant rats are resistant to insulin on glucose oxidation and that this insulin resistance is due to certain changes at the postreceptor sites.","subitem_description_type":"Abstract"}]},"item_10001_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"学位論文乙第227号「正常および糖代謝異常妊婦における赤血球インスリン受容体の解析」の参考論文その5","subitem_description_type":"Other"},{"subitem_description":"正常および糖代謝異常妊婦における赤血球インスリン受容体の解析","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本糖尿病学会"}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"日本糖尿病学会"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00166576","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-437X","subitem_source_identifier_type":"PISSN"}]},"item_10001_subject_21":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_10001_text_25":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_text_value":"Glucose Oxidation and Insulin Receptors in Isolated Adipocytes from Late Pregnant Rats"}]},"item_10001_text_43":{"attribute_name":"著作の一部","attribute_value_mlt":[{"subitem_text_value":"http://hdl.handle.net/10076/7538"}]},"item_10001_text_70":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Journal Article / 学術雑誌論文"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"豊田, 長康","creatorNameLang":"ja"},{"creatorName":"Toyoda, Nagayasu","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2017-02-18"}],"displaytype":"detail","filename":"AN001665760260801.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN001665760260801.pdf","url":"https://mie-u.repo.nii.ac.jp/record/7448/files/AN001665760260801.pdf"},"version_id":"4b0bb321-50aa-4ebe-b3b2-16ac7c0f0f7c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"グルコース酸化作用","subitem_subject_scheme":"Other"},{"subitem_subject":"インスリン受容体","subitem_subject_scheme":"Other"},{"subitem_subject":"脂肪細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"妊娠ラット","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"妊娠後期ラットの単離脂肪細胞におけるグルコース酸化作用およびインスリン受容体の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"妊娠後期ラットの単離脂肪細胞におけるグルコース酸化作用およびインスリン受容体の検討","subitem_title_language":"ja"}]},"item_type_id":"10001","owner":"15","path":["607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-03-22"},"publish_date":"2007-03-22","publish_status":"0","recid":"7448","relation_version_is_last":true,"title":["妊娠後期ラットの単離脂肪細胞におけるグルコース酸化作用およびインスリン受容体の検討"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-10-11T01:55:22.674195+00:00"}