@article{oai:mie-u.repo.nii.ac.jp:00007484, author = {李, 華植 and Lee, Hwa Sik and 間島, 雄一 and Majima, Yuichi and 坂倉, 康夫 and Sakakura, yasuo}, issue = {9}, journal = {Therapeutic Research}, month = {Sep}, note = {application/pdf, The purpose of this study was to investigate a simple, practical technique for the liquefying of the sticky mucus elements within nasal secretions, and to identify and count of the cells contained quantitatively. The subjects used in this study were mucopurulent nasal mucus from patients with chronic sinusitis. The following agents in varying concentrations were added to the nasal mucus, and were compared for their mucus-liquefying effect determined by the count, the population and the staining characteristics of the cells in nasal secretions: dithiothreitol (DTT), ethylenediaminetetraacetic acid tetrasodium salt(EDTA), propylene glycol(PG), serratio peptidase(SP), urea and Hanks' balanced salt solution (HBSS). HBSS was used as a control. For quantitative cytologic analysis smears were prepared from drug-treated nasal secretions with the use of a cytocentrifuge, and Wright-Giemsa stain was used on the obtained smears. The results indicate that 10 mM DTT is most effective for quantitative cytololgic study of mucopurulent nasal secretions.}, pages = {3059--3066}, title = {定量的鼻汁細胞診}, volume = {11}, year = {1990} }