{"created":"2023-06-19T11:39:28.144979+00:00","id":7532,"links":{},"metadata":{"_buckets":{"deposit":"a531cc4d-1b71-4860-b510-ce5c2811f648"},"_deposit":{"created_by":15,"id":"7532","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"7532"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00007532","sets":["334:606:607"]},"author_link":["18182","18183","18184","18185","18186"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-09-30","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"133","bibliographicPageStart":"125","bibliographicVolumeNumber":"26","bibliographic_titles":[{"bibliographic_title":"環境変異原研究"}]}]},"item_10001_description_19":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Reactive oxygen species generated by environmental factors, such as radiation, UV and chemicals can cause sequence-specific DNA damage and play important roles in mutagenesis and carcinogenesis. We have investigated sequence specificity of oxidative stress-mediated DNA damage by using ^<32>P-labeled DNA fragments obtained from the human c-Ha-ras-1, p53 and p16 genes. Free hydroxyl radicals cause DNA damage with no marked site specificity. Copper-hydroperoxo complex caused DNA damage at thymine, cytosine and guanine residues. ^1O_2 preferentially induces lesions at guanine residues. Benzoyloxyl radical specifically causes damage to the 5'-G in GG sequence ; this sequence is easily oxidized because a large part of the highest occupied molecular orbital of this radical is distributed on this site. Recently, we demonstrated that BP-7, 8-dione, a metabolite of carcinogenic benzo [a] pyrene (BP), strongly damaged the G and C of the 5'-ACG-3' sequence complementary to codon 273 of the p53 gene in the presence of NADH and Cu (II). BP-7, 8-dione also caused preferential double base lesion at 5'-TG-3' sequences. Since clustered DNA damage is poorly repaired, it is speculated that induction of the double base lesions in DNA might lead to activation of proto-oncogene or inactivation of the tumor suppressor gene. Therefore, oxidative DNA damage induced by BP-7,8-dione, especially double base lesions, may participate in the expression of carcinogenicity of BP in addition to DNA adduct formation. Here, we discuss the mechanisms of sequence-specific DNA damage including clustered DNA damage in relation to mutagenesis and carcinogenesis.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"日本環境変異原学会"}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"日本環境変異原学会","subitem_rights_resource":"本文データは学協会の許諾に基づきCiNiiから複製したものである"}]},"item_10001_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN1006217X","subitem_source_identifier_type":"NCID"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0910-0865","subitem_source_identifier_type":"PISSN"}]},"item_10001_subject_21":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_10001_text_25":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_text_value":"Mechanism of clustered DNA damage induced by oxidative stress and its role in mutagenesis and carcinogenesis(the symposium 2 \"Mechanism of DNA damege and mutation\" at the 32nd JEMS annual meeting, 2003.)"}]},"item_10001_text_41":{"attribute_name":"シリーズ","attribute_value_mlt":[{"subitem_text_value":"http://ci.nii.ac.jp/naid/110001708215/"}]},"item_10001_text_70":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Journal Article / 学術雑誌論文"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"及川, 伸二","creatorNameLang":"ja"},{"creatorName":"Oikawa, Shinji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"18182","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大西, 志保","creatorNameLang":"ja"},{"creatorName":"Ohnishi, Shiho","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"18183","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"村田, 真理子","creatorNameLang":"ja"},{"creatorName":"Murata, Mariko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"18184","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"平工, 雄介","creatorNameLang":"ja"},{"creatorName":"Hiraku, Yusuke","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"18185","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"川西, 正祐","creatorNameLang":"ja"},{"creatorName":"Kawanishi, Shosuke","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"18186","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-18"}],"displaytype":"detail","filename":"30A6626.pdf","filesize":[{"value":"837.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"30A6626.pdf","url":"https://mie-u.repo.nii.ac.jp/record/7532/files/30A6626.pdf"},"version_id":"f2820be9-036c-4f2f-a450-c5172bb3e213"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"reactive oxygen species","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA damage","subitem_subject_scheme":"Other"},{"subitem_subject":"double base lesions","subitem_subject_scheme":"Other"},{"subitem_subject":"carcinogenesis","subitem_subject_scheme":"Other"},{"subitem_subject":"hotspot","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"酸化ストレスによるクラスターDNA損傷機構とその変異における役割(日本環境変異原学会第32回大会シンポジウム2「DNA損傷と変異のメカニズム」)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"酸化ストレスによるクラスターDNA損傷機構とその変異における役割(日本環境変異原学会第32回大会シンポジウム2「DNA損傷と変異のメカニズム」)","subitem_title_language":"ja"}]},"item_type_id":"10001","owner":"15","path":["607"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-03-03"},"publish_date":"2008-03-03","publish_status":"0","recid":"7532","relation_version_is_last":true,"title":["酸化ストレスによるクラスターDNA損傷機構とその変異における役割(日本環境変異原学会第32回大会シンポジウム2「DNA損傷と変異のメカニズム」)"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-10-11T05:06:51.629580+00:00"}