@techreport{oai:mie-u.repo.nii.ac.jp:00008052,
 author = {楠, 正人 and Kusunoki, Masato and 井上, 靖浩 and Inoue, Yasuhiro and 三木, 誓雄 and 問山, 裕二 and Toiyama, Yuji},
 month = {May},
 note = {application/pdf, 大腸癌幹細胞が発癌過程または浸潤及び転移過程で周囲間質と相互作用をしながら進展していく様を二光励起顕微鏡で生体内可視化すること試みた。大腸癌肝転移モデルの早期では、癌細胞と肝類洞内皮細胞との接着及び停止、癌細細胞に胞血小板が付着するtumor cell induced platelet aggregation、癌細胞と白血球との相互作用が観察できた。後期では癌細胞塊と周囲間質及び腫瘍血管が観察できた。RFP-HT29の5-FU抵抗性クローンは癌細胞の紡錘形変化と瀰漫性増殖への変化と周囲の著明な間質反応が確認できた。, We attempted to visualize in vivo dynamic interactions between cancer stem cells (CSCs) and their surrounding stromal cells during the process of carcinogenesis, tumor invasion and metastasis in colorectal cancer using two photon laser scanning microscopy (TPLSM). We hypothesized that residual cancer cells after chemoradiotherapy (CRT) may contain putative CSCs which show not only CRT-resistant property, but also epithelial-mesenchymal transition (EMT) phenotype. On liver metastasis model, dynamic tumor-host interactions including tumor-endothelial, tumor-platelet, and tumor-leukocyte interactions were observed in vivo real-time by TPLSM. Treatment resistant clones (made by repeated exposure of anti-cancer drug or irradiation) were inoculated into mice, and visualized their characteristics in vivo real-time using TPLSM. The small fraction of treatment resistant clones showed spindle shaped morphology. Their growth pattern was changed from localized growth to diffusely invasive growth., 2009年度～2013年度科学研究費補助金(基盤研究(B))研究成果報告書, 21390377},
 title = {２光子励起顕微鏡を用いた大腸癌化及び癌、周囲間質応答の新規確証提示},
 year = {2014}
}