{"created":"2023-06-19T11:39:51.177366+00:00","id":8056,"links":{},"metadata":{"_buckets":{"deposit":"456dd6bd-a548-4b8a-b417-5564002491a3"},"_deposit":{"created_by":15,"id":"8056","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"8056"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00008056","sets":["334:627:1692670356714"]},"author_link":[],"item_8_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-05-22","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"核酸代謝酵素（以下MTAP)の欠損は種々の癌で高頻度に認められる。一方、正常細胞・組織では本酵素活性は正常であり、この違いを利用した選択的治療法の開発が期待されている。本研究では、簡便な診断方法として、フローサイトメトリー法（FCM)による酵素欠損の診断法についての基礎的検討を行った。更に、MTAPプロモーターメチル化診断のための高速液体クロマト法を開発した。","subitem_description_type":"Abstract"},{"subitem_description":"Methylthioadeosine phosphorylase (MTAP) is frequently deficient in a variety of malignancies, although the enzyme is active in normal cells or tissues. This difference in enzyme activities can be exploited to design the tumor-specific chemotherapy. We tried to develop the diagnostic method of MTAP enzyme deficiency which can be easily applied to clinical samples compared to genetic diagnosis. Anti-MTAP monoclonal antibody which we generated worked well with certain cell lines, whereas it did not with other cell lines. This might be caused by the difference in the amount of MTAP protein expressed. Since MTAP deficiency is known to be caused by the gene deletion or promoter hypermethylation, we developed the rapid high-pressure liquid chromatography (HPLC) to detect methylated DNA. PCR products were amplified from the methyl sulfite-modified DNA and PCR products with single nucleotide substituions were separated by HPLC.","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2011年度～2013年度科学研究費補助金(基盤研究(C))研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"23590668","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_18":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_text_value":"Basic evaluation of diagnosis of nucleic acid metabolizing enzyme deficiency by flow cytometry"}]},"item_8_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"登, 勉","creatorNameLang":"ja"},{"creatorName":"NOBORI, Tsutomu","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-18"}],"displaytype":"detail","filename":"30K17526.pdf","filesize":[{"value":"105.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"30K17526.pdf","url":"https://mie-u.repo.nii.ac.jp/record/8056/files/30K17526.pdf"},"version_id":"132e3fd3-a62f-44c3-aa82-9fcc3a62d718"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"MTAP","subitem_subject_scheme":"Other"},{"subitem_subject":"酵素欠損","subitem_subject_scheme":"Other"},{"subitem_subject":"プリン代謝","subitem_subject_scheme":"Other"},{"subitem_subject":"フローサイトメトリー","subitem_subject_scheme":"Other"},{"subitem_subject":"選択的化学療法","subitem_subject_scheme":"Other"},{"subitem_subject":"コンパニオン診断薬","subitem_subject_scheme":"Other"},{"subitem_subject":"プロモーターメチル化","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"フローサイトメトリーによる核酸代謝酵素欠損症診断法の基礎的検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"フローサイトメトリーによる核酸代謝酵素欠損症診断法の基礎的検討","subitem_title_language":"ja"}]},"item_type_id":"8","owner":"15","path":["1692670356714"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2016-01-15"},"publish_date":"2016-01-15","publish_status":"0","recid":"8056","relation_version_is_last":true,"title":["フローサイトメトリーによる核酸代謝酵素欠損症診断法の基礎的検討"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-11-07T04:16:24.010598+00:00"}