{"created":"2023-06-19T11:39:52.554429+00:00","id":8088,"links":{},"metadata":{"_buckets":{"deposit":"8ff11504-984a-4d17-8da1-ce4c4df2add4"},"_deposit":{"created_by":15,"id":"8088","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"8088"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00008088","sets":["334:627:1692670376097"]},"author_link":["19895"],"item_8_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-05-29","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"テネイシン-C（TN-C）は虚血性心疾患などの炎症部位に一過性に高発現する。TN-Cを特異的に認識する抗体を用いて、分子診断薬として改良可能かどうかを検証した。ハイブリドーマのRNAから抗TN-C抗体のH鎖可変領域（VH）とL鎖可変領域（VL）遺伝子をクローニングし、重複伸長PCRを行い、VH-linker-VL遺伝子（scFv遺伝子）を大腸菌、in vitroセルフリー（RTS小麦胚芽）の発現系、哺乳類細胞株（HEK293）へそれぞれ遺伝子導入した。その結果、HEK293において最も多く発現量が得られた。準備が整い次第、心筋炎モデル動物に注入して病巣への集積を確認する予定である。","subitem_description_type":"Abstract"},{"subitem_description":"Tenascin-C (TN-C) is expressed transiently inflammatory regions in various cardiovascular diseases. We investigated the possibility that specific antibody against TN-C is useful for detecting cardiovascular diseases as a molecular diagnostic reagent. Heavy chain variable region (VH) and light chain variable region (VL) genes of anti-TN-C antibody were obtained from total RNA in hybridoma, and were performed overlap-extended PCR. Expression vector inserted VH-linker-VL gene (scFv) was used in E. coli, in vitro cell free (RTS wheat germ) and mammalian cell line (HEK293) expression systems. As a result, the antibody expression was highly effective in HEK293 cell line system. As soon as it’s ready, we are planning to experiments of antibody accumulation in foci of myocarditis model mice.","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2012年度～2014年度科学研究費補助金(若手研究(B))研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"24791291","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_18":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_text_value":"Establishment of diagnosis based on molecular targeting imaging of cardiovascular diseases by scFv antibody against Tenascin-C"}]},"item_8_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"下條, 尚志","creatorNameLang":"ja"},{"creatorName":"SHIMOJO, Naoshi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-18"}],"displaytype":"detail","filename":"30K17918.pdf","filesize":[{"value":"306.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"30K17918.pdf","url":"https://mie-u.repo.nii.ac.jp/record/8088/files/30K17918.pdf"},"version_id":"feee39b8-dd75-4d37-b6a5-e5925bb5f813"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ScFv型抗体","subitem_subject_scheme":"Other"},{"subitem_subject":"テネイシン-C","subitem_subject_scheme":"Other"},{"subitem_subject":"分子イメージング","subitem_subject_scheme":"Other"},{"subitem_subject":"虚血性心疾患","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"ScFv化抗テネイシンＣ抗体による心疾患の分子標的イメージング診断法の確立","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ScFv化抗テネイシンＣ抗体による心疾患の分子標的イメージング診断法の確立","subitem_title_language":"ja"}]},"item_type_id":"8","owner":"15","path":["1692670376097"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-01-13"},"publish_date":"2017-01-13","publish_status":"0","recid":"8088","relation_version_is_last":true,"title":["ScFv化抗テネイシンＣ抗体による心疾患の分子標的イメージング診断法の確立"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-11-07T04:40:45.441446+00:00"}