@article{oai:mie-u.repo.nii.ac.jp:00008097,
 author = {Tanaka, Koji and Morimoto, Yuhki and Toiyama, Yuji and Matsushita, Kohei and Kawamura, Mikio and Koike, Yuhki and Okugawa, Yoshinaga and Inoue, Yasuhiro and Uchida, Keiichi and Araki, Toshimitsu and Mizoguchi, Akira and Kusunoki, Masato},
 journal = {Journal of oncology},
 month = {Nov},
 note = {application/pdf, In vivo real-time visualization of the process of angiogenesis in secondary tumors in the same living animals presents a major
challenge in metastasis research.We developed a technique for intravital imaging of colorectal liver metastasis development in live
mice using two-photon laser scanning microscopy (TPLSM). We also developed time-series TPLSM in which intravital TPLSM
procedures were performed several times over periods of days to months. Red fluorescent protein-expressing colorectal cancer cells
were inoculated into the spleens of green fluorescent protein-expressing mice. First- and second-round intravital TPLSM allowed
visualization of viable cancer cells (red) in hepatic sinusoids or the space of Disse. Third-round intravital TPLSM demonstrated
liver metastatic colonies consisting of viable cancer cells and surrounding stroma with tumor vessels (green). In vivo time-course
imaging of tumor angiogenesis in the same living mice using time-series TPLSM could be an ideal tool for antiangiogenic drug
evaluation, reducing the effects of interindividual variation.},
 title = {In vivo time-course imaging of tumor angiogenesis in colorectal liver metastases in the same living mice using two-photon laser scanning microscopy},
 volume = {2012},
 year = {2011}
}