{"created":"2023-06-19T11:39:53.957297+00:00","id":8121,"links":{},"metadata":{"_buckets":{"deposit":"92b9ee56-d4f3-47ef-b293-e688afc71fce"},"_deposit":{"created_by":15,"id":"8121","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"8121"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00008121","sets":["631:636:1692684513889"]},"author_link":["20147","20148"],"item_8_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-05-01","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"平成18年度では、肺胞上皮細胞と肺胞マクロファージを用いて、炎症性サイトカイン産生・分泌に対するプロテインS(PS)影響を検討した。さらに、リポポリサッカライド(LPS)による肺傷害モデルマウスに対するPSおよび活性化プロテインC(APC)とPSの併用の抑制効果を検討した。\n1)炎症性サイトカインの発現に及ぼすPSの影響:野生型(C57BL/6)マウス由来肺胞マクロファージは気管支肺胞洗浄液から分離する。人由来の肺胞上皮細胞(A549)・単球(THP-1)の細胞株も実検に用いた。各々の細胞はPSとリポポリサッカライド(LPS)で培養し、TNF-α、IL-1β、MCP-1、IL-6などの炎症性サイトカインの産生をELISA法とPCR法で検討した。その結果、PSはLPS刺激による肺胞マクロファージ、A549細胞、THP-1細胞からの炎症性サイトカインの産生を抑制した。\n2)急性肺傷害に対するAPC+PSの効果:C57BL/6野生型マウスにLPS(5mg/kg)を気管内に投与し、急性肺傷害モデルを作製した。LPS(5mg/kg)の気管内投与1時間前にAPC(1mg/kg)、PS(1mg/kg)、APC+PSまたは生理食塩を腹内投与し、24時間後に血液、気管支肺洗浄液中のTNF-α、IL1-βなどの炎症性サイトカイン量、好中球、肺胞マクロファージの数、組織学的変化で急性肺傷害の程度をグループ間に比較検討する。その結果、PS投与群とAPC+PS投与群では生理食塩投与群にくらべ、気管支肺胞洗浄液中の炎症性サイトカイン、細胞浸潤が有意に低下した。","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"平成18〜19年度科学研究費補助金(基盤研究(C)(2))研究成果報告書","subitem_description_type":"Other"},{"subitem_description":"津","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"18590846","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"田口修"}]},"item_8_subject_16":{"attribute_name":"日本十進分類法","attribute_value_mlt":[{"subitem_subject":"490","subitem_subject_scheme":"NDC"}]},"item_8_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"田口, 修","creatorNameLang":"ja"}],"nameIdentifiers":[{"nameIdentifier":"20147","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Gabazza, Esteban","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"20148","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-18"}],"displaytype":"detail","filename":"39K8028.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"39K8028.pdf","url":"https://mie-u.repo.nii.ac.jp/record/8121/files/39K8028.pdf"},"version_id":"e3a89d79-db2b-4318-9aac-c9fa29129ac3"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"プロテインS","subitem_subject_scheme":"Other"},{"subitem_subject":"炎症性サイトカイン","subitem_subject_scheme":"Other"},{"subitem_subject":"血液凝固系","subitem_subject_scheme":"Other"},{"subitem_subject":"活性化プロテインC","subitem_subject_scheme":"Other"},{"subitem_subject":"肺胞マクロファージ","subitem_subject_scheme":"Other"},{"subitem_subject":"血液凝固制御機構","subitem_subject_scheme":"Other"},{"subitem_subject":"急性肺傷害","subitem_subject_scheme":"Other"},{"subitem_subject":"感染症","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"プロテインC制御系因子の併用療法による急性肺傷害の抑制効果の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"プロテインC制御系因子の併用療法による急性肺傷害の抑制効果の解析","subitem_title_language":"ja"}]},"item_type_id":"8","owner":"15","path":["1692684513889"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2009-01-15"},"publish_date":"2009-01-15","publish_status":"0","recid":"8121","relation_version_is_last":true,"title":["プロテインC制御系因子の併用療法による急性肺傷害の抑制効果の解析"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-08-30T04:38:13.518701+00:00"}