{"created":"2023-06-19T11:39:55.237077+00:00","id":8151,"links":{},"metadata":{"_buckets":{"deposit":"c01c755e-f261-42f5-921a-757a254b0add"},"_deposit":{"created_by":15,"id":"8151","owners":[15],"pid":{"revision_id":0,"type":"depid","value":"8151"},"status":"published"},"_oai":{"id":"oai:mie-u.repo.nii.ac.jp:00008151","sets":["631:636:1692684649876"]},"author_link":["20261"],"item_8_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-06-05","bibliographicIssueDateType":"Issued"}}]},"item_8_description_14":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"デキサメサゾン受性急性リンパ性白血病細胞株から耐性株を誘導した。耐性細胞ではBim mRNAやタンパク質発現が低下した。またグルココルチコイド受容体αサブユニット（GRα）発現も耐性株では低下した。Bim発現を抑制するmiR 17-92クラスターやGRα発現を抑制するmiR142-3pは耐性株で発現が上昇した。miR142-3p mimicとinhibitor導入では細胞の増殖や生存への影響を認めなかった。GR発現に応じたmimic/inhibitor投与時期の検討や導入効率改善が必要であると考えられた。治療中の新鮮白血病MRD細胞からは充分なmRNA量が得られるよう系の改良を行っている。","subitem_description_type":"Abstract"},{"subitem_description":"Dexamethazone resistant acute lymphoblastic leukemia cell lines were established from sensitive cell lines. Both mRNA and protein expression of Bim showed marked reduction, and the expression of GR alpha subunit also decreased. Expressions of both miR17-92 cluster which inhibits Bim expression and miR142-3p which inhibits GR alpha expression indicated remarkable increase. Administration of mimic (agonist) or inhibitor for miR142-3p to the resistant cell lines did not indicate alteration in cell proliferation or survival. It was supposed that adequate time of mimic/inhibitor administration according to GR alpha expression should be considered, and also efficacy of mimic/inhibitor transfection should be improved. We could not get sufficient mRNA dose from freshly isolated minimal residual cells in bone marrow from leukemia patients, so still we are trying to improve the experimental procedure.","subitem_description_type":"Abstract"}]},"item_8_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"2011年度～2013年度科学研究費補助金(基盤研究(C))研究成果報告書","subitem_description_type":"Other"}]},"item_8_description_64":{"attribute_name":"科研費番号","attribute_value_mlt":[{"subitem_description":"23591539","subitem_description_type":"Other"}]},"item_8_publisher_30":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"三重大学"}]},"item_8_text_18":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_text_value":"Characteristics of leukemic stem cell activity in minimal residual disease of pediatric acute lymphoblastic leukemia"}]},"item_8_text_65":{"attribute_name":"資源タイプ（三重大）","attribute_value_mlt":[{"subitem_text_value":"Kaken / 科研費報告書"}]},"item_8_version_type_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"出口, 隆生","creatorNameLang":"ja"},{"creatorName":"DEGUCHI, Takao","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"20261","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-02-18"}],"displaytype":"detail","filename":"39K17528.pdf","filesize":[{"value":"180.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"39K17528.pdf","url":"https://mie-u.repo.nii.ac.jp/record/8151/files/39K17528.pdf"},"version_id":"b1ad4ece-ab9e-41ad-85c6-07f782f5f02a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"小児血液学","subitem_subject_scheme":"Other"},{"subitem_subject":"白血病","subitem_subject_scheme":"Other"},{"subitem_subject":"アポトーシス","subitem_subject_scheme":"Other"},{"subitem_subject":"微小残存病変","subitem_subject_scheme":"Other"},{"subitem_subject":"microRNA","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"小児急性リンパ性白血病の微小残存病変を用いた白血病幹細胞特性解析の試み","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"小児急性リンパ性白血病の微小残存病変を用いた白血病幹細胞特性解析の試み","subitem_title_language":"ja"}]},"item_type_id":"8","owner":"15","path":["1692684649876"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2016-01-15"},"publish_date":"2016-01-15","publish_status":"0","recid":"8151","relation_version_is_last":true,"title":["小児急性リンパ性白血病の微小残存病変を用いた白血病幹細胞特性解析の試み"],"weko_creator_id":"15","weko_shared_id":-1},"updated":"2023-11-13T01:07:10.564167+00:00"}