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Engineering of a Cellulolytic Bacterium and a Lignocellulose-degrading Enzyme for Utilization of Cellulosic Biomass
http://hdl.handle.net/10076/15151
http://hdl.handle.net/10076/151511f9d5a20-6a4d-4e20-a996-60f77f1c9cee
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
2015DI002.pdf (5.8 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||
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| 公開日 | 2016-06-28 | |||||||
| タイトル | ||||||||
| タイトル | Engineering of a Cellulolytic Bacterium and a Lignocellulose-degrading Enzyme for Utilization of Cellulosic Biomass | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
| 資源タイプ | doctoral thesis | |||||||
| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| 著者 |
市川, 俊輔
× 市川, 俊輔
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| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | For sustainable development of human societies, we should produce the products such as biofuel from renewable and environmentally-friendly materials instead of petroleum. Cellulosic biomass is a candidate material for the biofuel production. To achieve commercial biofuel production from cellulosic biomass, the significant cost reduction is required. Here I proposed the biofuel production strategy in which a cellulolytic bacterium degrades cellulosic biomass and produces fuel compounds, simultaneously. Enzymatic degradation of cellulosic biomass is the costly process in the biofuel production. To understand the detail feature of the enzymatic degradation of cellulosic biomass, one of the most powerful lignocellulose-degrading enzyme produced from a cellulolytic bacterium was characterized. The lignocellulose-degrading enzyme harbor a carbohydrate-binding module, which is used to determine the binding character of the enzyme in this study. I report the binding characters at the molecular level: the carbohydrate-binding module binds to various components of cellulosic biomass such as crystalline and non-crystalline form of cellulose, and xyloglucan. Few researchers have reported the examples of engineering of lignocellulose-degrading enzymes for improvement of the degradation ability of cellulosic biomass so far. Here, I show that the engineered lignocellulose-degrading enzyme with the replacement of carbohydrate-binding modules changed the specificity of the activity. Additionally, I also showed that the engineered enzyme could efficiently degrade a cellulosic biomass, milled corn hull, than the original enzyme, suggesting the possibility that the replacement of carbohydrate-binding modules will be one of the ways to improve the degradation ability of lignocellulose-degrading enzymes for cellulosic biomass. Some cellulolytic bacteria produce water-soluble fuel compounds such as ethanol. To collect water-soluble fuel compounds from the culture broth, a distillation step is required, which also significantly increases the cost of biofuel production. In contrast to the water-soluble fuel compounds, it is expected that water-insoluble compounds such as higher alcohols are more easily collected from the culture broth by phase separation due to their insolubility in water. Here I report that the engineered cellulolytic bacterium produced higher alcohols from cellulose in which the costly processes, the supplementation of lignocellulose-degrading enzymes and distillation of fuel compounds, were eliminated. These results in the characterization and the engineering of lignocellulose-degrading enzyme and cellulolytic bacteria in this study will be basic information of process developments for the further cost reduction of the biofuel production, resulting the establishment of economic biofuel production from cellulosic biomass. | |||||||
| 言語 | en | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 本文 / Laboratory of Food Chemistry and Biomass Graduate School of Regional Innovation Studies, Mie University, Japan | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 83p | |||||||
| 書誌情報 |
発行日 2016-01-01 |
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| フォーマット | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | application/pdf | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| その他の言語のタイトル | ||||||||
| その他のタイトル | セルロース系バイオマス利用に向けたセルロース系バイオマス分解細菌とその分解酵素の改変 | |||||||
| 言語 | ja | |||||||
| 出版者 | ||||||||
| 出版者 | 三重大学 | |||||||
| 学位名 | ||||||||
| 学位名 | 博士(学術) | |||||||
| 学位授与機関 | ||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||
| 学位授与機関識別子 | 14101 | |||||||
| 学位授与機関名 | 三重大学 | |||||||
| 学位授与年月日 | ||||||||
| 学位授与年月日 | 2016-03-25 | |||||||
| 学位授与番号 | ||||||||
| 学位授与番号 | 甲学術第1787号 | |||||||
| 資源タイプ（三重大） | ||||||||
| 値 | Doctoral Dissertation / 博士論文 | |||||||
