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エキソソーム型トロンボモジュリンによる敗血症治療
http://hdl.handle.net/10076/00020911
http://hdl.handle.net/10076/00020911ad17a799-f6c3-46a1-87c0-f92923f330ff
名前 / ファイル | ライセンス | アクション |
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2022RP0054 (294.4 kB)
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Item type | 報告書 / Research Paper(1) | |||||
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公開日 | 2022-11-28 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | エキソソーム型トロンボモジュリンによる敗血症治療 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Treatment of sepsis with exosomal thrombomodulin | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | トロンボモジュリン | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 敗血症 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Fc融合蛋白 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | エキソソーム | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||
資源タイプ | research report | |||||
著者 |
川本, 英嗣
× 川本, 英嗣× 江口, 暁子× 島岡, 要× 高娃, 阿栄 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | トロンボモジュリンの細胞外ドメインであるTMD123-Fc、またはドメインを欠いたTM-Fcタンパク質(TMドメイン12-Fc、TMドメイン23-Fc)を作成し、ELISAによりin vitroでのフィブロネクチンとの結合を調べた。トロンボモジュリンのレクチン様ドメインは、トロンボモジュリンの細胞外ドメインのフィブロネクチンへの結合に必須であることがわかった。TMD123-FcとTMD12-Fcの両方が、ヒト乳癌由来細胞株のβ1インテグリンとフィブロネクチンとの結合を阻害することを発見した。 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We created an extracellular domain of thrombomodulin, TMD123-Fc, or domain deletion TM-Fc proteins (TM domain 12-Fc, TM domain 23-Fc) and examined their bindings to fibronectin in vitro by ELISA. The lectin-like domain of thrombomodulin was found to be essential for the binding of the extracellular domain of thrombomodulin to fibronectin. Using a V-well cell ad-hesion assay or flow cytometry analysis with fluorescent beads, we found that both TMD123-Fc andTMD12-Fc inhibited the binding between β1 integrin of human breast cancer-derived cell lines and fibronectin. Furthermore, TMD123-Fc and TMD12-Fc inhibited the binding of activated integrins to fibronectin under shear stress in the presence of Ca²⁺ and Mg²⁺ but not under strong integrin-activation conditions in the presence of Mg²⁺ without Ca²⁺. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 2018年度~2020年度科学研究費補助金(基盤研究(C))研究成果報告書 | |||||
書誌情報 |
発行日 2021-05-15 |
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フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
出版者 | ||||||
出版者 | 三重大学 | |||||
出版者(ヨミ) | ||||||
ミエダイガク | ||||||
科研費番号 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 18K08917 | |||||
資源タイプ(三重大) | ||||||
Kaken / 科研費報告書 |