High cellulase activity was detected in the extract of marine gammarid amphipods inhabiting sea weed beds. The cellulase was partially purified by ammonium sulfate precipitation and successive column chromatographies. One of the two fractions separated by Sephadex G-100 gel chromatography was C₁-cellulase (correspond to avicelase, E. C. 3. 2. l. 91), which hydrolyzed more insoluble cellulose, avicel and filterpaper, than soluble cellulose, carboxymethylcellulose. The other was Cx-enzyme (correspond to CMCase, E. C. 3. 2. 1.4), which cleaved more soluble carboxymethylcellulose. Both enzymes had pH optimum of around 5.5, and was fairly stable in a pH region of 5.0 to 8.0 for 20 hr at 30℃ and was completely stable below 40℃ for 30 min-incubation at pH 7.0. C₁-and Cx-enzymes had molecular weights of approximately 34,000 and 26,000, respectively. These cellulase were estimated to originate from gammarids in the sbsence of cellulolytic symbiotic bacteria.
雑誌名
三重大学生物資源学部紀要 = The bulletin of the Faculty of Bioresources, Mie University
巻
3
ページ
87 - 95
発行年
1990-02-28
ISSN
0915-0471
書誌レコードID
AN10073846
フォーマット
application/pdf
著者版フラグ
publisher
日本十進分類法
468
その他のタイトル
On the Existence of Cellulase in Marine Gammarid Amphipods Inhabiting Seaweed Beds