The production of lytic enzyme by strain V 37 was investigated using polypeptone-yeast extract medium or chemically defined medium under various culture conditions.
Strain V 37 grew well and also produced lytic enzyme at pH5.0,6.0,7.0 and 8.0 in polypeptone-yeast extract medium containing 0.5 mole /l of NaCl.
The high yield of lytic enzyme was observed in the media containing NaNO₃ (0.1-1.0 mole/l) or NaSCN (0.2-0.3 mole/l). Chloride (Cl⁻) and nitrate (NO₃⁻）ions were more effective than thiocyanate (SCN⁻), dihydrogenphosphate (H₂PO₄⁻) and sulfate (SO₄²⁻) ions for the production of lytic enzyme. The elevated MgCl₂ in the presence of NaCl rather inhibited the production of lvtic enzyme.
Strain V 37 could not grow in a chemically defined medium proposed by MACLEOD (1968) during 24 hours of cultivation.
雑誌名
三重大学水産学部研究報告 = Bulletin of the Faculty of Fisheries, Mie University
Studies on Marine Bacteria Producing Lytic Enzymes-Ⅴ Culture Condition for the Production of Lytic Enzyme
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