We investigated the effects of poly (ADP - ribose) polymerase (PARP) inhibitors and
NAD on the apoptosis induced by Actinomycin D in human promyelomonocytic
leukemia cells HL - 60. As the results, the apoptosis was repressed by various PARP
inhibitors especially by 3 - aminobenzamide ( 3 – ABA ), which is a potent inhibitor forPARP reaction. On the contrary, 3-ABA was no more inhibitory in a cell-free apoptosissystem. This result suggests that the inhibitory effect of 3 - ABA is effective only before apoptotic cytosol is made up in the cells, i.e., in an early stage of the apoptotic process. But NAD inhibited the apoptosis in the same system depending on NAD concentration. So the inhibition of apoptosis by NAD may be concerned in a later step of apoptosis.
雑誌名
三重大学生物資源学部紀要 = The bulletin of the Faculty of Bioresources, Mie University
Effects of poly (ADP-ribose) polymerase inhibitors and NAD on the apoptosis induced by actinomycin D in human leukemia cells HL-60
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