| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2008-11-12 |
| タイトル |
|
|
タイトル |
Unphosphorylated twitchin forms a complex with actin and myosin that may contribute to tension maintenance in catch |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
catch contraction |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
twitchin |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
ABRM |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
myosin |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
actin |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Funabara, Daisuke
Hamamoto, Chieko
Yamamoto, Koji
Inoue, Akinori
Ueda, Miki
Osawa, Rika
Kanoh, Satoshi
J., Hartshorne David
Suzuki, Suechika
Watabe, Shugo
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Molluscan smooth muscle can maintain tension over extended periods with little energy expenditure, a process termed catch. Catch is thought to be regulated by phosphorylation of a thick filament protein, twitchin, and involves two phosphorylation sites, D1 and D2, close to the N and C termini, respectively. This study was initiated to investigate the role of the D2 site and its phosphorylation in the catch mechanism. A peptide was constructed containing the D2 site and flanking immunoglobulin (Ig) motifs. It was shown that the dephosphorylated peptide, but not the phosphorylated form, bound to both actin and myosin. The binding site on actin was within the sequence L10 to P29. This region also binds to loop 2 of the myosin head. The dephosphorylated peptide linked myosin and F-actin and formed a trimeric complex. Electron microscopy revealed that twitchin is distributed on the surface of the thick filament with an axial periodicity of 36.25 nm and it is suggested that the D2 site aligns with the myosin heads. It is proposed that the complex formed with the dephosphorylated D2 site of twitchin, F-actin and myosin represents a component of the mechanical linkage in catch. |
| 書誌情報 |
The journal of experimental biology
巻 210,
号 Pt24,
p. 4399-4410,
発行日 2007-12-01
|
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0022-0949 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00697490 |
| PubMed番号 |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
18055628 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1242/jeb.008722 |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 日本十進分類法 |
|
|
主題Scheme |
NDC |
|
主題 |
464 |
| 出版者 |
|
|
出版者 |
Cambridge University Press |
| 関係URI |
|
|
|
関連名称 |
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2219545 |
| 関係URI |
|
|
|
関連名称 |
http://jeb.biologists.org/cgi/content/full/210/24/4399 |
| ノート |
|
|
値 |
出版者版電子ジャーナルあり |
| 資源タイプ（三重大） |
|
|
値 |
Journal Article / 学術雑誌論文 |
50A7882.pdf (4.9 MB)
