WEKO3
アイテム
Molecular Characterization of the Subgroup Differentiation in Binucleate Rhizoctonia AG-Ba, causal agents of rice sclerotial diseases.
http://hdl.handle.net/10076/11644
http://hdl.handle.net/10076/11644a13b9c88-1741-4eed-a248-e2d8cc767778
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
60C15225.pdf (150.0 kB)
|
|
| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2011-11-07 | |||||
| タイトル | ||||||
| タイトル | Molecular Characterization of the Subgroup Differentiation in Binucleate Rhizoctonia AG-Ba, causal agents of rice sclerotial diseases. | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Rhizoctonia AG-Ba | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | rDNA-ITS region | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | RFLP analysis | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | Sequence analysis | |||||
| キーワード | ||||||
| 言語 | en | |||||
| 主題Scheme | Other | |||||
| 主題 | DNA Fingerprinting | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
Urushizaki, Shingo
× Urushizaki, Shingo× Inagaki, Kimiharu× Kobayashi, Issei× Arakawa, Masao |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Rhizoctonia spp. consists of genetically incompatible strain groups (anastomosis group, AG) distinguished by lack of anastomosis between hyphae of strains. In recent years, nucleotide sequences of rDNA-ITS region are widely used for the analysis of molecular diagnoses, classifications of DNA barcode and evolutions of plant pathogenic fungi. In this study, six different taxa of genus Rhizoctonia, includes three genus Thanatephorus, Waitea and Ceratobasidium on the teleomorph, of rice and weed sclerotial fungi, were classified with PCR-RFLP band patterns. Eight isolates of bincleate Rhizoctonia AG-Ba, the gray sclerotial fungus, showed three banding patterns, Type I (4 isolates), Type II (2 isolates) and a mixed-banding type (2 isolates). To determine whether the mixed-banding type arose from genome mixing between Type I and II, we cloned PCR products of ITS regions include 5.8S rDNA sequence. Twelve sequences derived from each isolates were sequenced from both directions and multiply aligned. The cluster analysis based on the multiple alignment showed that sequences derived from each isolate divided into two clades. The sequences derived from mixed-banding type isolates were divided into both two clades, revealing these isolates caused from genome crossing between Type I and II. To confirm this interpretation, microsatellite fingerprints of 54 isolates of binucleate Rhizoctonia AG-Ba were investigated and the fingerprint patterns were analyzed with cluster analysis. Unexpectedly, fingerprint patterns derived from 54 isolates were divided into three clades, unlike results of ITS sequencing. The result of cluster analysis likely to cause by unique banding patterns of the mixed type isolates, although banding patterns of few isolates showed mixed character of Type I and II. Because of superior information of microsatellite fingerprinting, the results lead us to interpretation that Rhizoctonia AG-Ba isolates should be classified into at least three subgroups. |
|||||
| 書誌情報 |
Proceedings of the Second International Workshop on Regional Innovation Studies : (IWRIS2010) 号 2, p. 13-16, 発行日 2011-10-01 |
|||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | Graduate School of Regional Innovation Studies, Mie University | |||||
| 資源タイプ（三重大） | ||||||
| 値 | Departmental Bulletin Paper / 紀要論文 | |||||
