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Nucleotide sequence diversity of rDNA internal transcribed spacers extracted from conidia and cleistothecia of several powdery mildew fungi
http://hdl.handle.net/10076/2607
http://hdl.handle.net/10076/2607ae0836e3-b2fb-4faa-943a-dcbe6891c988
名前 / ファイル | ライセンス | アクション |
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本文 (133.2 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||
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公開日 | 2007-03-20 | |||||||||
タイトル | ||||||||||
言語 | en | |||||||||
タイトル | Nucleotide sequence diversity of rDNA internal transcribed spacers extracted from conidia and cleistothecia of several powdery mildew fungi | |||||||||
言語 | ||||||||||
言語 | eng | |||||||||
キーワード | ||||||||||
主題Scheme | Other | |||||||||
主題 | internal transcribed spacers | |||||||||
キーワード | ||||||||||
主題Scheme | Other | |||||||||
主題 | nucleotide sequence | |||||||||
キーワード | ||||||||||
主題Scheme | Other | |||||||||
主題 | phylogeny | |||||||||
キーワード | ||||||||||
主題Scheme | Other | |||||||||
主題 | powdery mildew | |||||||||
資源タイプ | ||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
資源タイプ | journal article | |||||||||
著者 |
Hirata, Tetsuya
× Hirata, Tetsuya
× Takamatsu, Susumu
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抄録 | ||||||||||
内容記述タイプ | Abstract | |||||||||
内容記述 | An improved protocol, including DNA extraction with Chelex, two amplifications with a nested primer set, and DNA purification by electrophoresis, made it possible to analyze nuclear rDNA sequences of powdery mildew fungi using at most several hundred conidia or 20 cleistothecia. Nucleotide sequence diversity of the nuclear rDNA region containing the two internal transcribed spacers (ITS1 and ITS2) and 5.8S rRNA gene derived from conidia and cleistothecia was investigated for four kinds of powdery mildew fungi including two isolates of the same species. The results showed that the nucleotide sequences of the nuclear rDNA region were highly conserved between the teleomorph and the anamorph. Thus, the nucleotide sequence data obtained from either developmental stage can be used for phylogenetic studies of powdery mildew fungi. The nucleotide sequences of the 5.8S rRNA genes of the four species were highly conserved, but those of their ITS regions were variable. This suggests that the nuclear rDNA region is not suitable for phylogenetic studies of distantly related powdery mildew fungi, because too much sequence diversity exists within the ITS, end too little phylogenetic information is contained within the 5.8S rRNA gene. However, the ITS region will be useful for phylogenetic comparison of closely related species or intraspecies. | |||||||||
書誌情報 |
Mycoscience 巻 37, 号 3, p. 283-288, 発行日 1996-07-01 |
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ISSN | ||||||||||
収録物識別子タイプ | PISSN | |||||||||
収録物識別子 | 1340-3540 | |||||||||
書誌レコードID | ||||||||||
収録物識別子タイプ | NCID | |||||||||
収録物識別子 | AA11003436 | |||||||||
DOI | ||||||||||
関連タイプ | isVersionOf | |||||||||
識別子タイプ | DOI | |||||||||
関連識別子 | 10.1007/BF02461299 | |||||||||
フォーマット | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | application/pdf | |||||||||
著者版フラグ | ||||||||||
出版タイプ | AM | |||||||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||
日本十進分類法 | ||||||||||
主題Scheme | NDC | |||||||||
主題 | 474 | |||||||||
出版者 | ||||||||||
出版者 | Mycological Society of Japan | |||||||||
関係URI | ||||||||||
関連名称 | http://www.springerlink.com/content/4471u37457574k84/fulltext.pdf | |||||||||
ノート | ||||||||||
出版者版電子ジャーナルあり | ||||||||||
資源タイプ(三重大) | ||||||||||
Journal Article / 学術雑誌論文 |