| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2009-07-13 |
| タイトル |
|
|
タイトル |
Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Nakagawa, Tsuyoshi
Kurose, Takayuki
Hino, Takeshi
Tanaka, Katsunori
Kawamukai, Makoto
Niwa, Yasuo
Toyooka, Kiminori
Matsuoka, Ken
Jinbo, Tetsuro
Kimura, Tetsuya
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
We developed a new series of binary vectors useful for Gateway cloning to facilitate transgenic experiments in plant biotechnology. The new system, Gateway Binary Vectors (pGWBs) realized efficient cloning, constitutive expression using the cauliflower mosaic virus (CaMV) 35S promoter and the construction of fusion genes by simple clonase reaction with an entry clone. The reporters employable in this system are GUS, sGFP, LUC, EYFP, and ECFP. The tags available are 6xHis, FLAG, 3xHA, 4xMyc, 10xMyc, GST, T7-epitope, and tandem affinity purification (TAP). In total, 13 kinds of reporter or tag were arranged and were almost applicable to both N- and C-fusions. The pGWBs could be used for many purposes, such as promoter::reporter analysis, observation of subcellular localization by the expression of proteins fused to a reporter or tag, and analysis of protein-protein interaction by copurification and immunodetection experiments. The pGWBs were constructed with modified pBI101 containing a CaMV35S promoter-driven hygromycin phosphotransferase (HPT) gene as the second selection marker. We also constructed pGWBs with the marker HPT driven by the nopaline synthase promoter. By using the pGWB system, the expression of tagged proteins, and the localization of GFP-fused proteins were easily analyzed. Moreover, tissue-specific and inducible gene expression using a promoter was also monitored with pGWBs. It is expected that, the pGWB system will serve as a powerful tool for plasmid construction in plant research. |
| 書誌情報 |
Journal of bioscience and bioengineering
巻 104,
号 1,
p. 34-41,
発行日 2007-07-25
|
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
1389-1723 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11307678 |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 日本十進分類法 |
|
|
主題Scheme |
NDC |
|
主題 |
471 |
| 出版者 |
|
|
出版者 |
Society for Bioscience and Bioengineering, Japan |
| 資源タイプ（三重大） |
|
|
値 |
Journal Article / 学術雑誌論文 |
本文 (3.1 MB)
