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Mesenchymal Stromal Cell Secretome Up-Regulates 47kDa CXCR4 Expression, and Induce Invasiveness in Neuroblastoma Cell Lines
http://hdl.handle.net/10076/00019145
http://hdl.handle.net/10076/000191454d220a1b-fe23-462a-9610-9bdeb691c5dd
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
2015DM002 (5.8 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||
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| 公開日 | 2020-07-30 | |||||||
| タイトル | ||||||||
| タイトル | Mesenchymal Stromal Cell Secretome Up-Regulates 47kDa CXCR4 Expression, and Induce Invasiveness in Neuroblastoma Cell Lines | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
| 資源タイプ | doctoral thesis | |||||||
| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| 著者 |
Vipin, Shankar
× Vipin, Shankar
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| 著者（ヨミ） | ||||||||
| 姓名 | ビピン, シャンカー | |||||||
| 言語 | ja | |||||||
| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Neuroblastoma accounts for 15% of childhood cancer deaths and presents with metastatic disease of the bone and the bone marrow at diagnosis in 70% of the cases. Previous studies have shown that the Mesenchymal Stromal Cell (MSC) secretome, triggers metastases in several cancer types such as breast and prostate cancer, but the specific role of the MSC factors in neuroblastoma metastasis is unclear. To better understand the effect of MSC secretome on chemokine receptors in neuroblastoma, and its role in metastasis, we studied a panel of 20 neuroblastoma cell lines, and compared their invasive potential to wards MSC-conditioned-RPM I(mRPMI) and their cytokine receptor expression profiles. Western blot analysis revealed the expression of multiple CXCR4 isoforms in neuroblastoma cells. Among the five major isoforms, the expression of the 47kDa isoform showed significant correlation with high invasiveness. Pretreatment with mRPMI up-regulated the expression of the 47kDa CXCR4 isoform and also increased MMP-9 secretion, expression of integrin α3 and integrin β1 , and the invasive potential of the cell; while blocking CXCR4 either with AMD3100, a CXCR4 antagonist ,or with an anti-47kDa CXCR4 neutralizing antibody decreased the secretion of MMP-9, the expression of integrin α3 and integrin β1, and the invasive potential of the cell. Pretreatment with mRPMI also protected the 47kDa CXCR4 isoform from ubiquitination and subsequent degradation. Our data suggest a modulatory role of the MSC secretome on the expression of the 47kDa CXCR4 isoform and invasion potential of the neuroblastoma cells to the bone marrow. | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 本文/Department of Pediatrics, Mie University Graduate School of Medicine | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 21p | |||||||
| 書誌情報 |
発行日 2015-07-15 |
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| DOI | ||||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | 10.1371/journal.pone.0120069 | |||||||
| フォーマット | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | application/pdf | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 出版者 | ||||||||
| 出版者 | 三重大学 | |||||||
| 出版者（ヨミ） | ||||||||
| 値 | ミエダイガク | |||||||
| 学位名 | ||||||||
| 学位名 | 博士(医学) | |||||||
| 学位授与機関 | ||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||
| 学位授与機関識別子 | 14101 | |||||||
| 学位授与機関名 | 三重大学 | |||||||
| 学位授与年月日 | ||||||||
| 学位授与年月日 | 2015-07-15 | |||||||
| 学位授与番号 | ||||||||
| 学位授与番号 | 甲医学第1738号 | |||||||
| ノート | ||||||||
| 資源タイプ（三重大） | ||||||||
| 値 | Doctoral Dissertation / 博士論文 | |||||||
